Characterisation of putative gene CDK7 and its possible role in endocycles and lar...
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Cdk7 gene characterisation and analysis of its possible role in the endocycles of Rhynchosciara americana.
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| Author(s): |
Adam Arai Martens
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2012-04-20 |
| Examining board members: |
Fábio Siviero;
Margareth de Lara Capurro Guimaraes;
Lyria Mori
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| Advisor: | Fábio Siviero |
| Abstract | |
CDKs are proteins responsible for activation and progression of cell cycle and also work on the activation and elongation of transcription. Among them, CDK7 acts in both functions, phosphorylating cell cycle CDKs and as a part of general transcription factor TFIIH as its catalytic subunit. From an EST library of salivary gland, constructed with mRNAs present during the period when the beginning of the last polyteny replicative cycle occurs, it was found only few messages directly related to cell cycle, corresponding to 3.11% of the ESTs. Among them, it was found Cdc2-like and Cdk7; therefore, it was performed the characterisation of Cdk7 gene and the analysis of its role during the larval development of Rhynchosciara americana. Cdk7 gene presents 4 exons, more than in vertebrates. Complete mRNA sequence was obtained via RACE, presenting 1230 bases and an 1020 bases ORF. Expression profiles were determined by RT-PCR and Western blots. Posttranslational modifications were analysed by 2D immunoblots. Its mRNA and protein expression profiles presented variations during cell cycle and between the studied tissues; immunoblots showed the presence of one phosphorylation and possible modifications on the side chain of some amino acids. The study of proteins related to cell cycle in this model is important for a better understanding of uncommon cell cycles in different insect tissues. (AU) | |
| FAPESP's process: | 09/11276-1 - Characterisation of putative gene CDK7 and its possible role in endocycles and larval development of Rhynchosciara americana |
| Grantee: | Adam Arai Martens |
| Support Opportunities: | Scholarships in Brazil - Master |