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| Author(s): |
Letusa Albrecht
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2008-06-24 |
| Examining board members: |
Gerhard Wunderlich;
Fábio Trindade Maranhão Costa;
Marcelo Urbano Ferreira;
Paulo Lee Ho;
Jeffrey Jon Shaw
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| Advisor: | Gerhard Wunderlich |
| Abstract | |
Herein, we studied the repertoire of multigenic families of var (DBLa domain), rif and stevor from brazilian isolates of Plasmodium falciparum. We showed that these multigenic families could be conserved among different isolates from Brazil, suggesting that the repertoire of variant genes can be limited in certain areas. We identified less polymorphism in stevor genes than in var or rif genes. We also demonstrated that var genes can be conserved over time in Brazilian isolates and identified the DBLa and DBLb domains from var genes associated with cytoadherence to CHOICAM1 in Rondonian isolates. ELISA assays showed that these DBLbICAM domains were recognized in a heterogeneous fashion. By flow cytometry, we demonstrated that ICAM1-adherent parasites were stronger recognized by plasma from symptomatic infections than from non-symptomatic infections. Finally, we profiled the var gene transcripts in 3D7 parasites adherent to different CHO cells by real time PCR. (AU) | |