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| Author(s): |
Vanessa Olzon Zambelli
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-07-06 |
| Examining board members: |
Yara Cury;
Rodrigo Alvaro Brandão Lopes Martins;
Carlos Amilcar Parada;
Gisele Picolo;
Giles Alexander Rae
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| Advisor: | Yara Cury |
| Abstract | |
This study aimed to evaluate the mechanisms involved in the increased analgesic efficacy of crotalphine (CRP), an opioid-like peptide, under inflammation prostaglandin E2 (PGE2) and tissue injury (chronic constriction injury/CCI). PGE2 (intraplantar) in rats, increases the genic and proteic expression of <font face=\"Symbol\">m- and <font face=\"Symbol\">k-opioid receptors in the dorsal root ganglia (DRG) and nerve paw (NP), when compared to naïve rats. CCI up-regulates the expression of <font face=\"Symbol\">m and <font face=\"Symbol\">d -opioid receptors in DRG and NP. In contrast, <font face=\"Symbol\">k-opioid receptors were down-regulated by CCI. Although PGE2 and CCI, per se, do not activate opioid receptors, CRP and opioid agonists lead to a higher activation of these receptors in NP slices under PGE2 or CCI sensitization or in DRG cells incubated with PGE2. Moreover, CRP activates ERK1/2 and JNK MAPKs pathways, <font face=\"Symbol\">k-receptor and PKC<font face=\"Symbol\">z-mediated, only when the cells were pre-incubated with PGE2. These data indicate that the expression and activation of opioid receptors are distinctly regulated by the presence of acute or chronic injury. (AU) | |
| FAPESP's process: | 07/00135-2 - Evaluation of expression and activation of opioid receptors after peripheral injury in rats |
| Grantee: | Vanessa Olzon Zambelli |
| Support Opportunities: | Scholarships in Brazil - Doctorate |