The adipocyte size contributes to the functional heterogeneity of white adipose tissue

Author(s)

Natalie Carolina de Castro

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2010-04-29

Examining board members

Fabio Bessa Lima; Miriam Helena Fonseca Alaniz; Jose Cipolla Neto

• Advisor: Fabio Bessa Lima

Abstract

The adipose tissue was considered to be a little active metabolic tissue, however, the most recent advances show that it plays an important function in the control of the energy homostasis. Based on this concept, this work aimed to characterize the morphology and metabolism of isolated adipocytes of three different depots, like: subcutaneous, periepididymal, retroperitoneal (SC, PE and RP) . The adipocytes of the different depots had been collected and submitted to morphologic analysis, metabolic assays and to analysis of the enzymes expressions involved on lipids and glucose metabolism. The results showed statistical significant difference on volume of the adipocytes among the three different depots (p< 0, 05), high lipogenic capacity of RP adipocytes and higher expression of proteins involved in lipogenic patways of SC adipocytes (p<0, 05). (AU)