Evaluation of PI3K expression and its interaction with angiotensin II on central autonomic nuclei of hypertensive animals.

Author(s)

Leila Buttler

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2011-07-15

Examining board members

Vagner Roberto Antunes; Eduardo Colombari; Thiago dos Santos Moreira

• Advisor: Vagner Roberto Antunes

Abstract

One cause of the hypertension is associated to changes in autonomic nuclei that control the cardiovascular reflexes. Among these nuclei are PVN, NST and RVLM. The ANG II, acting in these nuclei, contributes to the control of the AP. In vitro studies had demonstrated a signaling pathway of the ANG II that involves the activation of PI3K and Akt. In the present study we evaluated the PI3K activity via expression of p85 (PI3K) and p-Akt, through the immunoblotting of these central nuclei of SHR, WKY and Wistar. Moreover, we evaluated the interaction of PI3K to the cardiovascular effects of ANG II injected into the PVN of awake Wistar. The p85 expression was not different at all autonomic nuclei studied. However, the p-Akt expression was 1.5x higher in the PVN of SHR. Besides, the increase in the MAP elicited by the injection of ANG II into the PVN was reduced 5 min after the blockade of the PI3K, and restored 30 min later, suggesting that PI3K pathway is more activate at the PVN level of SHR and that the interaction between ANG II-PI3K is related to hypertension. (AU)

• FAPESP's process: 09/01827-0 - Evaluation of PI3K expression and its interaction with angiotensin on central autonomic nuclei of hipertensive animals
• Grantee: Leila Buttler
• Support Opportunities: Scholarships in Brazil - Master