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| Author(s): |
Vivian Almeida Paschoal
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2011-09-20 |
| Examining board members: |
Rui Curi;
Renata Gorjão;
Fabio Bessa Lima
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| Advisor: | Rui Curi |
| Abstract | |
The effects of eicosapentaenoic (EPA) and docosahexaenoic (DHA) on neutrophil function were compared. For this purpose, experiments were performed in isolated rat neutrophils. Cells with intact plasma membrane and DNA fragmentation were analyzed in order to determine the non-toxic concentrations of EPA and DHA. Production of H2O2 was increased in lower concentrations of DHA (50 <font face=\"Symbol\">mM compared to 100 <font face=\"Symbol\">mM of EPA). For production of O2 -, EPA stimulated at lower doses (12.5 <font face=\"Symbol\">mM compared to 100 <font face=\"Symbol\">mM of DHA). Both FAs increased synthesis and release of cytokines, CINC-2 and TNF-<font face=\"Symbol\">α, and did not change the production of IL-1<font face=\"Symbol\">b and nitric oxide after incubation of the cells for 18 hours. Only DHA increased the phagocytic capacity and fungicidal activity of neutrophils. These FAs showed distinct effects on cytokine production, phagocytosis capacity and fungicidal activity by neutrophils. For production of ROS, both EPA and DHA had similar actions, although at different concentrations. (AU) | |
| FAPESP's process: | 09/04028-1 - Comparative effect of eicosapentanoic (EPA) and docosahexaenoic (DHA) acids on neutrophil function |
| Grantee: | Vivian Almeida Paschoal |
| Support Opportunities: | Scholarships in Brazil - Master |