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| Author(s): |
Aurea Beatriz Martins Bach
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Física (IF/SBI) |
| Defense date: | 2010-04-05 |
| Examining board members: |
Said Rahnamaye Rabbani;
Tito Jose Bonagamba;
Antonio Martins Figueiredo Neto
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| Advisor: | Said Rahnamaye Rabbani |
| Abstract | |
Currently, Nuclear Magnetic Resonance spectroscopy in vitro has been extensively used to study biological tissues, acting as a powerful tool for chemical analysis. In particular, NMR of proton (1H) and phosphorus (31P) has been used to study muscle metabolism in animals with genetic diseases, such as mice with muscular dystrophy mdx, models for Duchenne muscular dystrophy (DMD). The DMD, which affects humans, is a recessive disorder linked to X-chromosome and occurs in 1 each 3,500 live births male. The DMD is characterized by the absence of dystrophin protein, which causes a progressive and rapid degeneration. Currently, the monitoring of disease progression and benefits of treatments is made by biopsy of muscle tissue. In this study, 1H NMR spectrum were acquired from samples of diaphragm and quadriceps muscle of mdx and control mice 3 or 6 months-old. Results were compared with histological analysis of the same tissues. The objective of this study is to monitor the normal development of the muscles of control animals and the progress of dystrophy in the muscles of mdx animals by analyzing the NMR spectra. Differences were found between the groups of animals comparing the integrals of the observed peaks, showing that dystrophy leads to alterations in several methabolic pathways in the mdx mouse. These results form the basis for studies of the disease in vivo, so then it can be possible to distinguish dystrophic muscles from healthy muscles and characterize different stages of the disease noninvasively. (AU) | |
| FAPESP's process: | 07/06237-1 - Nuclear Magnetic Resonance application to study mdx Muscular Distrophy in mice |
| Grantee: | Aurea Beatriz Martins Bach |
| Support Opportunities: | Scholarships in Brazil - Master |