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| Author(s): |
Anna Cecilia Duarte da Silva
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina (FM/SBD) |
| Defense date: | 2009-03-25 |
| Examining board members: |
Celso Ricardo Fernandes de Carvalho;
Sonia Jancar Negro;
Adenir Perini
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| Advisor: | Celso Ricardo Fernandes de Carvalho |
| Abstract | |
Prevalence of allergic respiratory diseases has increased and although its origin likely multifactorial, reduced physical activity seems to represent an important contributor. Objective: We hypothesize that an improvement in aerobic conditioning (AC) previous to OVA sensitization would reduce airway inflammation via either an imbalance Th1/Th2 pathway or regulatory cytokine (IL-10). Methods: BALB/c mice were divided in 4 groups (n=8): non-trained and non-sensitized (Control), AC and non-sensitized (AC), non-trained and OVA-sensitized (OVA), and AC and OVA (OVA+AC). AC was performed in a treadmill (8 weeks) followed by either OVA or saline sensitization. It was evaluated airway and peribronchial cell inflammation, specific OVA-IgE and -IgG1 titers, expression of Th1 (IL-2 and IFN-), Th2 (IL-4, IL-5, IL-13) or regulatory (IL-10) cytokines, PGE2 and airway remodeling. Peribronchial expression of eotaxin, RANTES, ICAM-1, VCAM-1, TGF- and VEGF was also evaluated. Results: AC and control groups had similar results in all evaluated outcome (p>0,05) OVA sensitization induced airway and peribronchial cell inflammation and remodeling and an increase in the IgG1 and IgE titers (p<0,05). An increase in the expression of Th2 but not Th1 cytokines was well as an increase in the expression of IL-10, ICAM-1, VCAM-1, RANTES, TGF- and VEGF was also observed. OVA+AC group presented a lower migration of eosinophils and lymphocytes to the airways and lower titer of IgG1 and IgE (p<0,05). OVA+AC group also presented lower expression of Th2 cytokines however Th1 levels were unchanged. A lower expression of IL-10, ICAM-1, VCAM-1, RANTES, TGF- and VEGF as well as airway remodeling was also observed in OVA+AC group (p<0,05). Conclusion: Our results suggest that improvement in aerobic conditioning inhibit allergic sensitization and inflammation (AU) | |