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EVALIATION OF WET BREWER’S GRAIN IN RUMINANTS DIETS TROUGHT NUCLEARS AND CORRELATED TECHNIQUES

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Author(s):
Sérgio Lucio Salomon Cabral Filho
Total Authors: 1
Document type: Master's Dissertation
Press: Piracicaba.
Institution: Universidade de São Paulo (USP). Centro de Energia Nuclear na Agricultura (CENA/STB)
Defense date:
Examining board members:
Adibe Luiz Abdalla; Solange Maria Gennari; Ivanete Susin
Advisor: Adibe Luiz Abdalla
Abstract

The study evaluated the wet brewer’s grain silage using six male, adults Santa Inês sheep. The animals had a permanent ruminal fistula, and were distributed in two 3x3 Latin Squares in a multiple designing, with six animals and three experimental periods. The treatments was constituted by three experimental diets, A, B and C where the ensiled brewer’s grain substituted the hay of Tifton 85 (Cynodon x Cynodon) in the following ratios: A - 100 % hay; B - 67 % of hay + 33 % of silage and C - 33 % of hay + 67 % of silage. The experimental diets were evaluated through the in vivo, in situ and in vitro techniques. The in vitro evaluation was the microbial incorporation technique using 32P as marker. Wet brewer’s grains was collected in different industries and different periods, and this material was submitted to chemical and neutron activation analysis. The in vivo evaluation indicated increases in the apparent digestibility of the CP, when the brewer’s grains were included in the diet (P<0,05), the values of the digestibility were 51,6; 66,1 and 66,3 % for the diets A, B and C respectively. Treatment B showed higher digestibility of OM (P<0,05) and lower digestibility of ADF fraction (P<0,05), when the silage increase in the diet. For the in situ evaluation, the values of MS degradability was higher when the levels of brewer’s grain increase in the diet (P<0,05), the positive linear regression was obtained in accordance with the equation: p = 0,155n + 53.866; R2 = 0,922. In the study of the incorporation of the 32P, the microbial synthesis was evaluated with the amounts of nitrogen incorporation (Ni) in the microbial mass. Experimental diets did not present a significant difference (P>0,05) of Ni values, that was around of 4,60; 4,62 and 5,03 mg Ni incorporated per g of fermented dry matter, for the diets A, B and C respectively. The silage process of the wet brewer’s grain was considered an efficient form of storage during the studied period. The used evaluation showed that the brewer’s grains presented a similar characteristics with the forage source and it was considerate a good roughage feed, even so in the in situ evaluation the rates of DM disappearance were higher than of Tifton hay. Reductions (P>0,05) in the voluntary intake of the animals were obtained when the brewer’s grain was included in higher amounts than 33 % of DM. Toxic elements presence, As, Hg and Cd were not identified in the composition of the brewer’s grain when analysed by neutron activation analysis. (AU)