Study of fatty acids metabolism in Pseudomonas putida aiming to modulate monomer composition of biodegradable elastomer.

Author(s)

Sonia Regina da Silva Queiroz

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2008-04-30

Examining board members

Luiziana Ferreira da Silva; Luiz Antonio Gioielli; Marilis do Valle Marques; Patrícia Locosque Ramos; Elisabete Jose Vicente

• Advisor: Luiziana Ferreira da Silva

Abstract

Different strategies were applied to modulate the composition of biodegradable elastomeric polyhydroxyalkanoates (PHA) accumulated by Pseudomonas from fatty acids and plant oils, in order to improve the applications of this material, mainly by unsaturated monomer insertion. PHA composition varied both with fatty acid type and fatty acids ratio in mixtures supplied. Analysis of genome sequences revealed two fadH (encoding 2,4-dienoyl-CoA hydratase) copies in Pseudomonas aeruginosa and only one in other Pseudomonas species. The number of fadH copies in the genome was related to the higher oxidation of unsaturated fatty acids. Transposon-induced mutants affected on unsaturated fatty acids metabolism were obtained, some of them showing higher efficiency to incorporate unsaturated monomers do the PHA. Cloning and sequencing of transposon-disrupted DNA regions allowed to identify the genes affected in those mutants. (AU)

• FAPESP's process: 03/11795-2 - Study of fatty acids metabolism in Pseudomonas putida aiming to modulate monomer composition of biodegradable elastomer
• Grantee: Sonia Regina da Silva Queiroz
• Support Opportunities: Scholarships in Brazil - Doctorate