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| Author(s): |
Bruna Felicio Milazzotto Maldonado Porchia
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2010-02-04 |
| Examining board members: |
Luis Carlos de Souza Ferreira;
Carlos Augusto Pereira;
Mauricio Martins Rodrigues
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| Advisor: | Luis Carlos de Souza Ferreira |
| Abstract | |
The development of vaccines against human papillomavirus (HPV) represents an important alternative to control the sexually transmitted infection and cervical cancer. In this paper we explored a novel vaccine strategy against tumors induced by HPV-16 using the E7 protein obtained after genetic fusion to the glycoprotein D of herpes virus type 1, a protein with adjuvant properties for T lymphocytes. The recombinant protein gDE7 was expressed in bacteria and in insect cells and purified by affinity chromatography. The protein generated in bacteria was administered in soluble and insoluble forms as a vaccine in mice. The insoluble gDE7 gave 80% prophylactic protection for tumor growth, the gDE7 that kept the soluble form after refolding protected 100% of the animals under the same conditions. The therapeutic protection reached 30% of the animals. We also verified the neutralizing potential of sera generated against the HSV-1. The conditions for obtaining the proteins expressed in insect cells were also established. (AU) | |
| FAPESP's process: | 06/58525-8 - Construction and analysis of the prophylactic and therapeutic proprieties of a vaccine against HPV-16 associated tumors |
| Grantee: | Bruna Felício Milazzotto Maldonado Porchia Ribeiro |
| Support Opportunities: | Scholarships in Brazil - Master |