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| Author(s): |
Natália de Souza Nunes
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2012-01-25 |
| Examining board members: |
Fabíola Attié de Castro;
Arlete Aparecida Martins Coelho Castelo;
Elvira Maria Guerra Shinohara
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| Advisor: | Fabíola Attié de Castro |
| Abstract | |
Polycythaemia vera (PV) is a clonal disorder characterized by an accumulation of normal red and white cells, platelets, and their progenitors in absence of a definable stimulus. Despite the advances in PV diagnosis and the description of the mechanisms involved in disease establishment its pathogenesis remains unclear. The apoptosis deregulation might have a role in PV physiopathology. Fully understanding the basic apoptotic pathway and its potential regulation by microRNA in PV patients cells might unveil targets for manipulation, which may be translated into novel therapies for disease.The aims of this study were to avaluate in leukocytes and CD34+ cells: (1) The microRNA expression whose RNA target are associated with apoptosis regulation (2) Correlation between microRNA expression and the mRNA levels of anti and pro-apoptotic family Bcl-2 expression and death receptors; (3) Correlation between microRNA expression and the clinical data of patients: hemoglobin concentration, hematocrit and JAK2 percentage. Patientes with Polycythemia Vera shows increased expression of microRNAs 29c, 16, 21, 26a, 130b and let-7d and 34c and 15a decrease in peripheral leukocytes; incresead expression of miRs 29c, 16 and 21, decrease in miR130b and let-7d expression in CD34+ cells; deregulation in pro and anti-apoptotic gene expression in peripheral leukocytes with increase in a1, mcl-1 and decrease in bcl-2, ciap-2, bax and fas-L, deregulation in pro and anti-apoptotic gene expression in CD34+ cells with increased expression of fas, bid, mcl-1, bcl-xl and c-flip and decreased expression of bik. Decreased in proteic expression of BCL-2 in peripheral leukocytes of patients when compared to controls. Correlation between de deregulated expression of microRNA and their genes target and those with hematological parameters. Lymphocytes from PV patients shows higher resistance to apoptosis than controls when induced by: Actinomicin, etoposide, cytarabine and cycloheximide. In conclusion the data suggest the involvement of microRNA in apoptosis regulation and the involvement of apoptosis machinery in the PV pathophysiology. These results will contribute for a better understanding of PV pathophysiology and will be useful for the discover of future therapies. (AU) | |
| FAPESP's process: | 10/01756-3 - MicroRNAs expression on CD34+ cells and leukocytes in Polycythemia Vera. |
| Grantee: | Natália de Souza Nunes |
| Support Opportunities: | Scholarships in Brazil - Master |