The Influence of different culture mediums conditions in chemical profile and cytotoxic activity of two Streptomyces strains

Author(s)

Noemi Jacques Vieira

• Document type: Master's Dissertation
• Press: Ribeirão Preto.
• Institution: Universidade de São Paulo (USP). Faculdade de Ciências Farmacêuticas de Ribeirão Preto (PCARP/BC)
• Defense date: 2011-06-10

Examining board members

Monica Tallarico Pupo; Hosana Maria Debonsi; Ana Helena Januário

• Advisor: Monica Tallarico Pupo

Abstract

Abstract Microorganisms are prolific producers of bioactive natural products. Most of innovative pharmaceuticals that have useful clinical importance have been isolated from from microorganisms, such as antibiotics, anticancer drugs, immunosuppressive drugs and lipid-lowering agents. Most of the drugs of microbial origin are produced by actinomycetes. The close association of micro-organisms with plants, insects, crustaceans or marine organisms may induce the production of bioactive substances. These natural products may confer adaptive advantages to the environment and to the host organism. Marine and transitional areas such as mangroves are still under explored for chemical and pharmacological studies of both soil microorganisms and symbiotic microorganisms. In this sense, this work aimed a chemical study of two actinobacteria strains, Streptomyces sp. Act1 and Streptomyces sp. Act2, guided by cancer cells cytotoxicity assays. Both microorganisms were isolated from the shell of the mangrove crab Ucides cordatus, collected in Cananéia, SP. These microorganisms showed antagonistic activity against the pathogenic fungus Aspergillus niger, confirming the potential for production of bioactive substances. Microorganisms were cultured in different culture media to assess the potential for growth, chemical profile of extracts and cytotoxic activity of extracts and subfractions. The culture media were chosen using liquid (ISP-2) and solid medium (rice). The effect of addition of saline water in the culture media has also been evaluated. The microorganisms developed adequately in different culture media. The fractions of extracts obtained from the experiments showed characteristics of polar subfractions obtained, requiring the use of sephadex LH-20 and reverse phase as chromatographic stationary phases. Cytotoxic assays of the extracts against cancer cell lines showed the great potential of the strains under study, mainly Streptomyces sp. Act2. grown in the presence of seawater. (AU)

• FAPESP's process: 09/01963-1 - Bioactive natural products from two Spreptomyces sp. associated to the mangrove crab
• Grantee: Noemi Jacques Vieira
• Support Opportunities: Scholarships in Brazil - Master