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| Author(s): |
Bruna Gabriela dos Santos Kotake
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | Ribeirão Preto. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) |
| Defense date: | 2012-10-19 |
| Examining board members: |
João Paulo Mardegan Issa;
Valeria Paula Sassoli Fazan;
Vanessa de Souza Mello
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| Advisor: | João Paulo Mardegan Issa |
| Abstract | |
Alcoholism is considered a reducer for new bone formation, may leading to osteometabolic disturbance, considering that the rhBMP-2 is a morphogenetic protein known to play an important role in the bone healing processes. The aim of this study was to investigate the action of alcoholism and its effect on the repair of bone defects (DO) performed in rat calvaria after the rhBMP-2 application, pure or combined with a collagen matrix. We used 80 rats divided into eight groups, each one with a waiting period for sacrifice of 4 and 6 weeks after the bone defect (5mm) delineation in the rat skull. The groups were divided into: G1) water \"ad libitum\" + DO, G2) alcohol \"ad libitum\" + DO, G3) water + DO + 5μg pure rhBMP-2, G4) alcohol + DO + 5μg pure rhBMP-2, G5 ) DO + water + collagen sponge carrier, G6) alcohol + DO + collagen sponge carrier, G7) and water + DO + 5μg rhBMP-2/collagen sponge, G8) alcohol + DO + 5μg of rhBMP-2/collagen sponge. The radiographic data were submitted to statistical analysis Kruskal-Wallis and Dunn\'s Multiple, histological data to analysis of variance (ANOVA) and Tukey test. Radiographically, it was found after 6 weeks that the groups treated with rhBMP-2, independently of the carrier use and ethanol administration, more new bone formation (p<0.05), at 4 weeks it was not found statistical difference. For immunohistochemical analysis, ostecalcin (OC), and bone sialoprotein (BPS) were predominant in groups treated with rhBMP-2. For histological quantification of collagen type I fibers, statistical difference between groups was found (p<0.05) with the increasing of these fibers in the groups treated with rhBMP-2 combined with collagen sponge; and quantitative by stereology, aiming to calculate the volume of new bone, it was found higher values for the groups treated with rhBMP-2 pure or combined to the carrier; but for the groups treated with ethanol, it was found higher bone formation in the groups treated with rhBMP-2 associated to the carrier in the periods of 4 and 6 weeks (p <0.001). It was concluded in this experimental model that the carrier was effective for rhBMP-2 delivery, even in the presence of ethanol. (AU) | |
| FAPESP's process: | 10/12452-5 - Assessment of the bone healing process in critical defects after application of the rhBMP-2 pure protein and / or combined in normal animals and under the effect of chronic alcoholism. |
| Grantee: | Bruna Gabriela dos Santos Kotake |
| Support Opportunities: | Scholarships in Brazil - Master |