Biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin

Author(s)

Aldo Enrique Del Carpio Perochena

• Document type: Master's Dissertation
• Press: Bauru.
• Institution: Universidade de São Paulo (USP). Faculdade de Odontologia de Bauru (FOB/SDB)
• Defense date: 2011-04-18

Examining board members

Clovis Monteiro Bramante; Flaviana Bombarda de Andrade; Paulo Henrique Weckwerth

• Advisor: Clovis Monteiro Bramante

Abstract

Introduction: The aim of this study is to evaluate the biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin. Methods: 120 bovine dentin specimens were infected intraorally using a removable orthodontic device. 30 samples were used for each irrigant solution; 2% Chlorhexidine, 1%, 2.5% and 5.25% of sodium hypochlorite (NaOCl). The solutions were used for 5, 15 and 30 minutes and 2 experimental volume 500µl and 1mL. The samples were stained using the acridine orange dye before and after the experiments and evaluated using a confocal microscope. The percentage of biofilm, isolated cells and no colonized dentin was measured using a grid system. Differences in the reduction or increase of the studied parameters was assessed using non-parametric methods ( P < 0.05). Results: The higher values of biofilm dissolution and clean dentin were found in the 30 minutes NaOCl groups and in the 5 and 15 minutes of 5.25% NaOCL. The use of 2% chlorhexidine solution does not improve the biofilm dissolution neither increases the cleaning of the dentin in comparison to the NaOCl solutions (P < 0.05). Conclusions: 2% chlorhexidine does not dissolve the biofilms. 30 minutes of sodium hypochlorite are necessary to have the higher values of biofilm dissolution and to increase the cleaning of the dentin independently of the concentration in comparison to the 5 min and 15 min contact time. (AU)

• FAPESP's process: 09/03217-5 - Biofilm dissolution and cleaning ability of different irrigant solutions on intraorally infected dentin
• Grantee: Aldo Enrique Del Carpio Perochena
• Support Opportunities: Scholarships in Brazil - Master