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| Author(s): |
Caio Yogi Yonamine
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-04-30 |
| Examining board members: |
Maria Tereza Nunes;
José Donato Júnior;
Rosana Cristina Tieko Mori
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| Advisor: | Maria Tereza Nunes |
| Abstract | |
HMB is a metabolite of leucine able to increase protein synthesis and/or reduce protein degradation. Studies carried out in our laboratory have shown that HMB-supplemented rats although normoglycemic, exhibited hyperinsulinemia, suggesting an insulin resistance status. In parallel, it was observed an increased activity of the GH-IGF-I axis, reinforcing the possibility of alterations on glucose sensitivity, since GH is recognized as being diabetogenic effects. The results demonstrate that HMB-supplemented rats, after glucose overload (0,75g/kg) present reduced glucose decay rate and increased plasma insulin levels (glucose tolerance test GTT), confirming the insulin resistance status. By western blotting, was detected reduced insulin action (liver and skeletal muscle), after insulin stimulus. No alterations were detected in retroperitoneal white adipose tissue. No significant alterations were detected on pSTAT5 and SOCS3 after HMB supplementation indicating that GH could act by another mechanism to induce insulin resistance. Additionally, was detected increased on mRNA and protein expression of hormone sensitive lipase (HSL), and plasma concentrations of non-esterified fat acids (NEFA) in HMB-supplemented rats, which could be a mechanism mediated by GH, contributing to insulin resistance. (AU) | |
| FAPESP's process: | 11/03254-8 - Molecular basis of effects of chronical administration of beta hydroxy beta methylbutyrate (HMB) on sensibility of insulin action on rats |
| Grantee: | Caio Yogi Yonamine |
| Support Opportunities: | Scholarships in Brazil - Master |