Mycobiota and occurrence of fumonisins in different genotypes of transgenic corn (Bt).

Author(s)

Vinicius de Morais Barroso

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-04-15

Examining board members

Benedito Correa; Eduardo Micotti da Gloria; Sarah Santos Gonçalves

• Advisor: Benedito Correa

Abstract

Fusarium verticillioides, fumonisin producer, is commonly associated with corn kernels. The proposed objective was to investigate the mycobiota and fumonisins in different genotypes of transgenic corn (Bt) and their respective controls. The mycobiota of grain, using the technique of direct plating, revealed the presence of several genera of fungi. The strains of Fusarium isolated were identified to species by classical and molecular methods. The average water activity in the hybrids was higher than 0.90. We observed greater fungal contamination in controls, with the exception of 2B710Hx. The relative frequency of Fusarium in different hybrids revealed lower contamination in transgenic, excepting AG8088VTPRO. F. verticillioides was the most common fungus. Analysis of fumonisins revealed fumonisin B1 in 92.5% of the samples and fumonisin B2 in 47.1%. No change was observed in the production of fumonisins in genotypes 2B710 and 30F35 (transgenic and non-transgenic). However AG8088 for genotype had lower production of fumonisin B1 in transgenic variety. (AU)

• FAPESP's process: 10/14264-1 - Mycoflora and occurrence of fumonisins in different genotypes of transgenic corn (Bt)
• Grantee: Vinicius de Morais Barroso
• Support Opportunities: Scholarships in Brazil - Master