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| Author(s): |
Maíra Mello Rezende Valle
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2009-08-28 |
| Examining board members: |
Angelo Rafael Carpinelli;
Rui Curi;
Mario Jose Abdalla Saad
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| Advisor: | Angelo Rafael Carpinelli |
| Field of knowledge: | Biological Sciences - Physiology |
| Indexed in: | Banco de Dados Bibliográficos da USP-DEDALUS; Biblioteca Digital de Teses e Dissertações - USP |
| Location: | Universidade de São Paulo. Biblioteca do Instituto de Ciências Biomédicas; T-ICB BMB QP34; V181ae |
| Abstract | |
Feeding animals with high fat diet containing lard causes obesity, insulin resistance and dysfunction of pancreatic beta cells. High fat diet induces oxidative stress and modulates NAD(P)H oxidase expression and activity in many tissues. This enzyme may be involved in many pathophysiological processes. The objective of this study was to evaluate the action of high fat diet on NAD(P)H oxidase activity and expression and if this fact can be connected to the beta cell dysfunction reported in the literature on this animal model. In pancreatic islets of rats fed the high fat diet apoptosis was reduced, glucose metabolism increased, insulin secretion elevated at high glucose, protein expression of NAD(P)H oxidase subunits reduced and the superoxide production was diminished. There was no difference between the groups for oxidative stress markers. It is possible that the enzyme has a role in the process of insulin secretion. Probably the islets are regulating their activity and function to compensate the deleterious effect of lard. (AU) | |