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| Author(s): |
Felipe Perozzo Daltoé
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Odontologia (FO/SDO) |
| Defense date: | 2013-12-02 |
| Examining board members: |
Andrea Mantesso Pobocik;
Luciana Corrêa;
Cesar Isaac;
Oswaldo Keith Okamoto;
Marinilce Fagundes dos Santos
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| Advisor: | Andrea Mantesso Pobocik |
| Abstract | |
Recent advances in cell sorting and characterization technics of stem cells have provided new insights and perspectives for clinical applications of this particular cell population. Most of the studies isolat stem cells based on the expression of cell surface markers and evaluate their stem cells-like proprieties by performing in vitro and in vivo assays. Here we isolated different populations of keratinocytes based on the expression of the epithelial stem cell markers neurotrophin receptor p75 (p75NTR) and transferrin receptor 1 (CD71). The co-expression of other epithelial stem markers such as integrins 6 and 1 was also assessed and in vitro functional assays such as colony-forming efficiency; long-term growth potential, in vitro epithelial reconstruction-capacity and response to damage and self-renew capacity. Our results showed that p75NTR+ve cells have better functional proprieties in most of the assays however they did not respond to damage nether presented self-renew capacity. Additionally, p75NTR-ve cells express higher percentage of other epithelial stem cell markers such as integrins 6 and 1 when compared to p75NTR-ve cells and the double staining (p75NTR+veCD71-ve) contributes to isolate a more enriched cell population based on the expression of the integrins. Nevertheless, independently of the cell population or of the amount of time in culture, cells have changed dramatically their phenotype in a way that the cell population initially p75NTR+ve lost the expression of the p75NTR and the negative population have gained it. (AU) | |
| FAPESP's process: | 10/06574-0 - Epithelial stem cells from oral mucosa: isolation, characterization and response to injury |
| Grantee: | Felipe Perozzo Daltoé |
| Support Opportunities: | Scholarships in Brazil - Doctorate |