The influence of Na,K-ATPase isoforms in ouabain signaling cascade against LPS induced NF-kB activation in glial cells.

Author(s)

Paula Fernanda Kinoshita

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2013-09-27

Examining board members

Cristoforo Scavone; Luis Eduardo Menezes Quintas; Ana Carolina Thomaz Takakura

• Advisor: Cristoforo Scavone

Abstract

Na,K-ATPase is a conserved membrane protein which maintains the osmotic balance in the cell by the hydrolysis of ATP. Ouabain (OUA) binds to Na,K-ATPase and it can activate signaling pathways. The a subunits of Na,K-ATPase have 4 isoforms which are distributed in a different pattern in the tissues. Glial cells have an important role in the response against injury and they also control inflammation. Some data have reported that OUA can protect against some types of injury. The aim of this study is to evaluate the role of a2 isoform in glial cells in response to OUA and LPS stimulus. We investigated the action of OUA and LPS in cell viability (LDH) and cell proliferation (MTT). LPS was used as a model of inflammation and one of our questions was if the treatment with OUA before LPS was capable of reduce the activation of the transcription factor NF-kB which is involved in inflammation. The pre-treatment with OUA decreased the NF-kB activation induced by LPS. We also silenced the a2 isoform in culture glial cells with iRNA. Taken together our data showed that OUA pretreatment reversed the NF-kB activation induced by LPS in primary cultures of glial cells from mice. Probably,the a2 isoform is related with some signaling pathway that interacts with the LPS pathway. (AU)

• FAPESP's process: 11/04327-9 - Changes in LPS induced gene expression by ouabain signaling on alpha1, alpha2 Na, K-ATPase in glial cells
• Grantee: Paula Fernanda Kinoshita
• Support Opportunities: Scholarships in Brazil - Master