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| Author(s): |
Patricia Ebersbach Silva
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-12-11 |
| Examining board members: |
Ubiratan Fabres Machado;
Maria Lucia Cardillo Correa Giannella;
Fernanda Ortis;
Marisa Passarelli;
Leticia Prates Roma
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| Advisor: | Ubiratan Fabres Machado |
| Abstract | |
High concentrations of saturated fatty acids trigger insulin resistance in skeletal muscle and endoplasmic reticulum stress (ER) is suggested in this process. This study aimed to investigate, in L6 muscle cells, the effects of palmitate treatment on ER stress pathways and activation of NF-kB, relating them to the impaired expression of GLUT4. The results showed that treatment with 0.75 mM of palmitate induced reduction in the amount of GLUT4 and Slc2a4. Stress markers such as GRP78, PERK/EIF2a and IRE1a/XBP-1/TRAF2 showed little activation. The transcription factor NF-kB were increased in protein content and mRNA. The binding activity of NF-kB to the promoter region of Slc2a4 showed increased independent from the phosphorylation of IKK. Finally, it was observed that palmitate represses the expression of Slc2a4 and that this fatty acid induces little activation of reticulum stress pathways in L6 cells. On the other hand, the involvement of NF-kB appears to be important to control this phenomenon, reducing the expression of Slc2a4 and impairing glucose homeostasis. (AU) | |
| FAPESP's process: | 10/09984-5 - ANALYSIS OF THE UNFOLDED PROTEIN RESPONSE (UPR) ON THE EXPRESSION OF GLUT4 PROTEIN IN MUSCLE CELLS TREATED WITH PALMITATE. |
| Grantee: | Patrícia Ebersbach Silva |
| Support Opportunities: | Scholarships in Brazil - Doctorate |