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| Author(s): |
Andressa Peres de Oliveira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2013-11-13 |
| Examining board members: |
Armando Morais Ventura;
Viviane Fongaro Botosso;
Dolores Ursula Mehnert;
Hugo Pequeno Monteiro;
Bryan Eric Strauss
|
| Advisor: | Armando Morais Ventura |
| Abstract | |
Human respiratory syncytial virus (HRSV) is one of the most important pathogens of the respiratory tract. We analyzed the interactions of viral nucleoprotein (N), phosphoprotein (P) and matrix (M) in HEK-293T. N interacts with the cellular proteins Hsp70, PRMT5 and WDR77; P interacts with Hsp70 and Tropomyosin; and M with Nucleophosmin and Tropomyosin. Each cellular gene was co-expressed with a viral gene in bacteria allowing co-precipitation of proteins. Hsp70 co-expression with N or P proteins confirmed that these interactions also occur in bacteria. Using a set of mutants we found that the N and P amino terminus, P carboxy terminus, and Hsp70 amino terminal domain participate in the interactions. The overexpression of Hsp70 by transfection resulted in stimulation of HRSV replication. Mice immunization with N and P showed that DNA vaccines were capable of inducing humoral and cellular response. In challenge assays it was possible to detect significant virus titer reduction in animals immunized with N, indicating its potential for a vaccine formulation. (AU) | |
| FAPESP's process: | 09/15400-9 - Functional Characterization of Human Respiratory Syncytial Virus Nucleoprotein and Phosphoprotein |
| Grantee: | Andressa Peres de Oliveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |