New role for galectin-1 as effector molecule of cytotoxic cells.

Author(s)

Tiago Clemente Machado

• Document type: Doctoral Thesis
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-03-18

Examining board members

Mauricio Martins Rodrigues; Niels Olsen Saraiva Câmara; Cristiane Damas Gil; Jose Maria Alvarez Mosig; Daniela Santoro Rosa

• Advisor: Joao Gustavo Pessini Amarante Mendes

Abstract

Exocytosis of secretory granules is the main effector mechanism of CD8+ T cells. In particular, little is known about CTLs lytic granules composition. Previous results from our group identified a few dozens of new proteins associated with these granules. Among them, we identified galectin-1. Literature reports the extracellular action of Gal-1. Initial data from our group suggested a new scenario for this protein, since Gal-1 was found inside cytotoxic granules. Here, we show by transmission electron and confocal laser scanning microscopy and cytotoxicity assays that Gal-1 has a role on CTL killing probably mediating the FAS-FASL pathway. We also show that Gal-1 is regulates the time of contact between APCs and TCD8+ lymphocytes, the activation of APCs and the proliferation of CD8 T cells. Taken together, our findings suggest a new scenario, in which Gal-1 is present in CTL granules and participates in cytotoxic effector response. (AU)

• FAPESP's process: 11/07444-6 - New role for galectin-1 as effector molecule of cytotoxic cells
• Grantee: Tiago Clemente Machado
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)