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| Author(s): |
Jamile Calil Silveira
Total Authors: 1
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| Document type: | Doctoral Thesis |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI) |
| Defense date: | 2014-04-22 |
| Examining board members: |
Maria Tereza Nunes;
Denise Pires de Carvalho;
Maria Luiza Morais Barreto de Chaves;
Murilo Vieira Geraldo;
Ileana Gabriela Sanchez de Rubio
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| Advisor: | Maria Tereza Nunes |
| Abstract | |
Pendrin is an iodide transporter in thyroid, encoded by the PDS gene. Since iodide is essential for the synthesis of T3 and T4, this study aimed to evaluate the iodine effect on pendrin gene expression and on the activity of its promoter. Male rats received an injection of saline or 2mg of NaI. After 30, 1, 24 and 48h the thyroids were removed for mRNA and protein analysis by Real Time PCR and Western Blot. Moreover, PCCl3 cells were treated or not with 10-3M NaI. After 30, 1, 24 and 48h the RNA/protein were isolated. The efflux was determined by measure of 125I in the culture medium. For the promoter analysis, it was inserted in pGL3-basic plasmid. The results indicated that the pendrin mRNA amount increased after iodide treatment. However, the promoter activity was unchanged. The protein expression and iodide efflux increased only after 24h of treatment. These data suggest an important role of pendrin for the autoregulation of the thyroid. (AU) | |
| FAPESP's process: | 10/05503-2 - EFFECT OF ACUTE ADMINISTRATION OF IODINE IN THE PENDRIN GENE EXPRESSION AND PROMOTER ACTIVITY: STUDY IN VIVO AND IN VITRO |
| Grantee: | Jamile Calil Silveira |
| Support Opportunities: | Scholarships in Brazil - Doctorate |