Pharmacological blockers of the renin-angiotensin system and the regulation of the metabolism in isolated fat cells.

Author(s)

Rennan de Oliveira Caminhotto

• Document type: Master's Dissertation
• Press: São Paulo.
• Institution: Universidade de São Paulo (USP). Instituto de Ciências Biomédicas (ICB/SDI)
• Defense date: 2014-05-21

Examining board members

Fabio Bessa Lima; Miriam Helena Fonseca Alaniz; Marcelo Augusto Christoffolete

• Advisor: Fabio Bessa Lima

Abstract

Recent data indicate a participation of the renina-angiotensin system (RAS) in metabolic process, due its local presence in tissues, like the adipose tissue, and suggests that these tissues could be targets of RAS blockers. Therefore, we have studied the possible effects of pharmacological RAS blockers in isolated fat cells. Therefore, fat cells were isolated of epididymal fat pad and treated with non toxic doses of Aliskiren or Captopril or Losartan. After 24 hours, the lipolytic, lipogenic and oxidative capacity were tested in their respective spontaneous and stimulated states. Also, gene expression of PPARg and RAS components were verified. The results showed Aliskiren increases the relation between oxidation and lipogenesis from glucose, whereas Captopril decreased glucose lipid incorporation, especially in glicerol fraction of triglyceride when insulin stimulus exist, and the Renin receptor gene expression. As a conclusion, Captopril and Aliskiren can directly modulate lipogenic and oxidative metabolism of isolated fat cells, but in a different way. (AU)

• FAPESP's process: 12/02054-8 - Pharmacological blockade of the renin-angiotensin system and the regulation of metabolism in isolated fat cells
• Grantee: Rennan de Oliveira Caminhotto
• Support Opportunities: Scholarships in Brazil - Master