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| Author(s): |
Paloma de Oliveira Tonietti
Total Authors: 1
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| Document type: | Master's Dissertation |
| Press: | São Paulo. |
| Institution: | Universidade de São Paulo (USP). Faculdade de Medicina Veterinária e Zootecnia (FMVZ/SBD) |
| Defense date: | 2013-03-12 |
| Examining board members: |
Fabio Gregori;
Paulo Eduardo Brandão;
Juliana Galera Castilho Kawai
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| Advisor: | Fabio Gregori |
| Abstract | |
The rotavirus and coronavirus are important viral agents associated with enteropathies in pigs, with implications for Animal Health, Public Health and Agribusiness. Nevertheless, there is little data about the detection and characterization of these viruses, especially the coronavirus. This study determined the occurrence of them on farrow-to-finish pig farms from 12 different cities in the São Paulo State, Brazil. For this purpose, three reactions, previously described, were performed: multiplex nested RT-PCR for simultaneous detection of two porcine coronavirus that can be found in the feces of these animals, the Transmissible Gastroenteritis Virus (TGEV) and the Porcine Epidemic Diarrhea Virus (PEDV), and group A rotavirus; a nested RT-PCR to investigate the occurrence of any member of the genus Coronavirus; and a RT-PCR for detection of the TGEV and Porcine Respiratory Coronavirus (PRCoV), which can also be observed in fecal samples of swine. For the first reaction, we used two pairs of primers targeting the S gene of TGEV (951bp and 793bp), two primers targeting to the M gene of PEDV (425bp and 291bp), and two primers targeting the NSP5 gene of group A rotavirus (317bp and 208bp). For the second reaction, four primers were used targeting to the RdRp gene of coronaviruses (251bp and 136bp). In the third reaction, a pair of primers was used targeting the S gene (886bp to TGEV and 205-214bp to PRCoV). This data showed that 40.37% of the total samples tested (88/218) and 91.6% of the cities (11/12 cities) were positive for rotavirus. Coronaviruses were not detected on farms examined in this study. The rotavirus positive stools were characterized based on PCR and nucleotide sequence analyses for the VP4 and VP7 genes. The VP7 complete nucleotide sequencing was obtained for one sample, and partial nucleotide sequencing for 34 (about 24.74% to 35.57% of coding region) and 23 (about 63.19% to 98.77% of coding region) samples for the VP4 and VP7 gene, respectively. The genotypes G3, G5 and G9 in combination with P[6], P[13] (and / or P[22]) and P[23] was found. Commercially available vaccine formulations include only the G4 and G5 rotavirus genotypes, which demonstrates a disadvantage in terms of protection of susceptible animals, whereas only one (G5) was detected in the animals analyzed in this study. The knowledge of this virus allows studies of the zoonotic and interspecies transmission of this microorganism and the strengthening of control and prophylactic measures directed to the agent. (AU) | |
| FAPESP's process: | 11/01563-3 - Ocurrencce and molecular characterization of porcine enteropathy-associated viruses in São Paulo State |
| Grantee: | Paloma de Oliveira Tonietti |
| Support Opportunities: | Scholarships in Brazil - Master |