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(EN) METHOD FOR PRODUCING AND PURIFYING HYBRID OR NON-HYBRID RECOMBINANT GLYCOPROTEIN HORMONES, HYBRID OR NON-HYBRID RECOMBINANT GLYCOPROTEIN HORMONES, EXPRESSION VECTORS AND USES OF THE RECOMBINANT GLYCOPROTEIN HORMONES

Document type:Patente
Inventor(s): BARUFFI, Marcelo Dias; ANDRADE, Camillo del Cistia; SILVA, Rubens Eduardo da; OLIVEIRA, Robinson Antonio Martins de; CALLEJON, Daniel Roberto
Applicant: Universidade de São Paulo (USP)
Deposit date: May 2016, 12
INPI register:
PCT/BR2016/000051
IPC: C12N 15/66 C07K 14/59 C12N 15/16 A61K 38/24 G01N 33/74 G01N 33/76
Abstract

(EN) The present invention relates to a method for producing hybrid or non-hybrid recombinant glycoprotein hormones, for example the recombinant equine chorionic gonadotropin (r-eCG), the hybrid recombinant chorionic gonadotropin, the recombinant thyroid-stimulating hormone (r-TSH), the recombinant luteinising hormone (r-LH), the luteinising hormone and the recombinant follicle-stimulating hormone (r-FSH). In addition, the present invention relates to the recombinant glycoprotein hormones comprising the equine α and β subunits, inter alia, the α subunit of mammals and equine β subunit, where the two subunits are fused in a simple chain, and chain-modifying agents, which hormones are easier to purify, more homogeneous, easier to produce on an industrial scale without using animals, in comparison with the wild glycoprotein hormone. Moreover, the present invention relates to the use of these hormones in assisted animal reproduction, ovulation induction, superovulation induction, follicle growth, oestrus induction, anoestrus reversal, puberty induction in animals, both with and without commercial interest.


FAPESP's process: 13/50869-3 - Recombinant equine chorionic gonadotropin (r-eCG) production in eukaryotic system for use in estrus synchronization protocols
Grantee:Camillo Del Cistia Andrade
Principal Investigator:Camillo Del Cistia Andrade
Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE