Abstract
Rates in vitro produced bovine embryos is related to efficiency of IVM procedure that intrinsically limits the developmental competence of the oocyte. Thus , by determining the mRNA expression of Cx43 , GDF -9 , BMP15 , OOSP1 and fibronectin in oocytes and GREM1 , PTGS2 , PFKP , VCAN , HAS2 and AREG genes in cumulus cells , this study was designed in order to adapt a culture system that e…