Ligia Ely Morganti Ferreira Dias

• Abstract

  Targeting immune responses to antigens that have been shown to be able of promoting protective immune responses may be a promising alternative for the development of safe and effective vaccines. Generally the presence of domains containing antigenic epitopes with the native conformation is important for the generation of neutralizing antibodies. For this reason, there is the need for the …

  Refolding of antigens synthesized as inclusion bodies in Escherichia coli for vaccine preparation, AP.R

• Abstract

  Endostatin (ES) inhibits angiogenesis, reducing tumor growth in animal models. However, it has low therapeutic effect in human clinical trials. BAX is a member of the BCL-2 family of proteins; its proapoptotic (BH3) domain interacts with other members of the family in the cytoplasm, to induce apoptosis. Here, we fused the BAX BH3 domain with murine ES, to enhance ES potency. Endothelial c…

  Improving the therapeutic potential of endostatin by fusing it with the bax bh3 death domain, PUB.ART

• Abstract

  Many relevant therapeutic proteins are found naturally in low concentrations. Escherichia coli is a very useful microorganism for recombinant protein production for structural and functional studies and for production of therapeutic proteins in high levels. However, the production of recombinant proteins often results in the accumulation of insoluble aggregates (inclusion bodies, IB) in b…

  Characterization of intermediate states of protein folding and obtainment of biofunctional proteins by high hydrostatic pressure, AP.R

(Only some records are available in English at this moment)

• Abstract

  Elucidating the mechanism by which a polypeptide folds from a wide range of unstructured states is an important step in protein engineering. To understand this mechanism it is important to structurally characterize all species (native, denatured state and intermediate states) produced along the folding pathway. High pressure promotes the stabilization of intermediary states of the folding…

  NMR studies at high pressure for characterization of protein intermediate states involved in the folding pathway, BE.EP.PD