graduate at Farmácia e Bioquímica from Faculdade de Ciências Farmacêuticas (1984), master's at Tecnologia Nuclear Básica from Instituto de Pesquisas Energéticas e Nucleares (1989) and ph.d. at Tecnologia Nuclear Básica from Instituto de Pesquisas Energéticas e Nucleares (1993). Has experience in Pharmacology, focusing on Pharmacology Molecular Biochemistry, acting on the following subjects: dna recombinant, human prolactin, expression, cho cells and endostatina. (Source: Lattes Curriculum)
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SARS-CoV-2 Nucleocapsid (N) is the most abundant viral protein expressed in host samples and is an important antigen for diagnosis. N is a 45 kDa protein that does not present disulfide bonds. Intending to avoid non-specific binding of SARS-CoV-2 N to antibodies from patients who previously had different coronaviruses, a 35 kDa fragment of N was expressed without a conserved motif in E. c…
ABSTRACTBackgroundProteins in inclusion bodies (IBs) present native-like secondary structures. However, chaotropic agents at denaturing concentrations, which are widely used for IB solubilization and subsequent refolding, unfold these secondary structures. Removal of the chaotropes frequently causes reaggregation and poor recovery of bioactive proteins. High hydrostatic pressure (HHP) and…
Enzymes from the thermolysin family are crucial factors in the pathogenesis of several diseases caused by bacteria and are potential targets for therapeutic interventions. Thermolysin encoded by the gene LIC13322 of the causative agent of leptospirosis, Leptospira interrogans, was shown to cleave proteins from the Complement System. However, the production of this recombinant protein usin…
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Proteínas recombinantes possuem um enorme potencial para o tratamento de doenças e vêm ganhando mais espaço na economia. Atualmente já movimentam um mercado de bilhões de dólares em todo o mundo, tornando-se um importante foco para companhias farmacêuticas e de biotecnologia. As bactérias Escherichia coli são as hospedeiras mais utilizadas para produzir essas proteínas. No entanto, a agre…
A necessidade de produção eficiente de proteínas recombinantes tem crescido e provavelmente continuará a crescer. As bactérias Escherichia coli são as hospedeiras mais utilizadas para produzir essas proteínas, mas resultam no acúmulo de depósitos de proteínas insolúveis e inativas no seu interior, denominados de corpos de inclusão. A expressão de proteínas em corpos de inclusão possui al…
Será realizada a purificação da prolactina humana (hPRL), hormônio da hipófise anterior, obtida através de técnicas de DNA recombinante em fluido periplásmico bacteriano de E.coli transformadas. Visando a purificação da hPRL serão analisadas e testadas as técnicas de precipitação com sulfato de amônio e cromatografias clássicas de interação hidrofóbica, troca iônica e exclusão molecular. …
Elucidating the mechanism by which a polypeptide folds from a wide range of unstructured states is an important step in protein engineering. To understand this mechanism it is important to structurally characterize all species (native, denatured state and intermediate states) produced along the folding pathway. High pressure promotes the stabilization of intermediary states of the folding…
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