Irene Fernandes

• Abstract

  Our goal in this project is to produce and characterize antibodies containing the single chain Fragment variable - scFv - anti-toxins from snake venoms, viewing to understand their mechanisms of action and a possible therapeutic alternative for snake bites of the genus Crotalus and Bothrops.Accidents caused by snake venoms were included in the list of Neglected Tropical Diseases of WHO. I…

  Production and characterization of scFv recombinant antibodies against toxins from Bothrops and Crotalus venoms, AP.R
• 10th Meeting of Pan American Section of International Society on Toxinology, AR.EXT
• Immunochemical and biological characterization of monoclonal antibodies against bap1, a metalloproteinase from bothrops asper snake venom., AR.EXT

(Only some records are available in English at this moment)

• Abstract

  Our goal in this project is to study the affinity of mutant human antibody scFv anti-crotoxin, seeking to understand the mechanisms of action of crotoxin and a possible alternative treatment for poisoning by the bite of snakes of the genus Crotalus. Crotalus poisoning, although accounting for only 8% of cases, it has the highest mortality. The venom has several toxins, crotoxin (CTX) prim…

  Affinity study of scFv human antibodies against crotoxin using mutants evoluted in silico, constructed and expressed in bacteria, BP.MS

• Abstract

  Administration of heterologous antibodies, generally produced in horses, has been the treatment of choice to persons who suffered accidents by snake venoms. However, this kind of therapy can induce adverse reactions. Small fragments of antibodies containing only the VH and VL domains, known as Fv (Fragment variable) or single chain Fv (scFv) were constructed by genetic engineer aiming to …

  Biological charaterization of original and mutant human recombinant antibodies (scFv) anti-crotoxin generated by site-directed mutagenesis, BP.IC