Abstract
The goal of this project is to develop a live attenuated vaccine against Francisella noatunensis subspecies orientalis (FNO) that can be applied to Nile tilapia (Oreochromis niloticus). The project will have five specific objectives: i) Produce at list one mutant strain of FNO deleting the wbtA, pilA, mglA, clpB, and tolC genes from a non-mutant Brazilian strain isolated in the Fish Insti…