Abstract
This Scientific Initiation project aims at studying the growth of the gram positive bacterium Bacillus megaterium, expressing the gene pac, of the enzyme penicillin G acylase.Kinetic parameters of cellular growth will be estimated, using the cultivation medium of Yang, 2007, as a basis.Shaker assays will be run, followed by experiments in an automated bioreactor of 2L. PGA, produced extra…