Maria Celia Bertolini

• Abstract

  Genome sequencing allows to perform regulatory studies in a deep way. In living cells genomes are not simply made of DNA and proteins, but rather of a large amount of regulatory proteins that dynamically interact with DNA and modulate gene expression. Our laboratory has been using the filamentous fungus Neurospora crassa as a model organism to study the molecular mechanisms that are invol…

  The filamentous fungus Neurospora crassa as a model organism for the functional characterization of proteins/transcription factors that regulate the carbohydrate metabolism, AP.TEM

• Abstract

  Genome sequencing allows performing regulatory studies in a deep way. In living cells genomes are not simply made of DNA, but rather of a large amount of regulatory proteins that dynamically interact with DNA and modulating gene expression. Using biochemical techniques coupled to mass spectrometry our laboratory identified proteins able to bind to the gsn promoter that codes glycogen synt…

  The Neurospora crassa filamentous fungus as a model organism for the identification and characterization of new proteins/transcription factors regulating the glycogen metabolism, AP.TEM
• Glycogen metabolism and stress response in Neurospora crassa: regulation of the gsn gene, AR.EXT

(Only some records are available in English at this moment)

• Abstract

  In Aspergillus nidulans fungus, the FlbC transcription factor is involved in the control of conidiation process through the activation of a cascade of genes. In Neurospora crassa, studies carried out in Bertolini´s laboratory showed that FLB-3, the FlbC ortholog, acts in the cascade of conidiation of the fungus, affecting the sexual and asexual development as well as the glycogen metaboli…

  Functional characterization of N. crassa FLB-3 transcription factor, BP.PD

• Abstract

  Using a collection of Neurospora crassa mutant strains in genes encoding protein kinases, a number of proteins were identified as regulators of the reserve carbohydrates glycogen and trehalose. Among these proteins, is the SNF-1 protein kinase already characterized in different organisms; in Saccharomyces cerevisiae this protein was described as involved in the control of the glycogen met…

  Functional characterization of protein kinases identified as regulators of glycogen metabolism in N. crassa, BP.PD

• Abstract

  The FlbC transcription factor was described, initially, in A. nidulans as a regulatory protein of the asexual development, triggering the process of conidiation through the activation of a cascade of genes and, so far, it has not been fully characterized at the functional level in other filamentous fungi. Previous results obtained with the fungi N. crassa showed that the FlbC ortholog pro…

  Functional characterization of N. crassa FLB-3 transcription factor, BP.PD

(Only some records are available in English at this moment)

• Abstract

  Metabolism of storage carbohydrate, such as glycogen, is under regulation of the circadian clock in mammals, and dysfunction of either glucose metabolism or clock regulation is associated with insulin resistance and diabetes. Glycogen is synthesized and degraded as a function of the organism metabolism status and the endogenous clock determines properties of this regulation. Regulation of…

  Connection studies between biological clock and glycogen metabolism regulation in Neurospora crassa, BE.EP.DR

• Abstract

  Eukaryotic gene expression control is determined by a complex transcriptional regulatory network, which can be described as a set of interactions among many different transcription factors and regulatory DNA sequences present in different genomes. All these interactions, combined with significant changes occurring simultaneously in chromatin structure, account for the competence in which …

  Identification of proteins and/or protein complexes that modulate gene expression in Neurospora crassa under heat stress, BE.EP.PD