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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Re-induction of desiccation tolerance after germination of Cedrela fissilis Vell. seeds

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Author(s):
Masetto, Tathiana E. [1] ; Faria, Jose M. [2] ; Fraiz, Ana C. R. [3]
Total Authors: 3
Affiliation:
[1] UFGD, Fac Ciencias Agr, BR-79804970 Dourados, MS - Brazil
[2] Univ Fed Lavras UFLA, Dept Ciencias Florestais, BR-37200000 Lavras, MG - Brazil
[3] Univ Fed Vicosa, BR-36570000 Vicosa, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Anais da Academia Brasileira de Ciências; v. 86, n. 3, p. 1273-1285, SEP 2014.
Web of Science Citations: 7
Abstract

This work aimed to characterize the re-induction of desiccation tolerance (DT) in germinated seeds, using polyethylene glycol (PEG 8000). Cell changes were investigated through cytological assays (cell viability and transmission electronic microscopy) as well as DNA integrity during loss and re-establishment of DT. The loss of DT was characterized by drying germinated seeds with different radicle lengths (1, 2, 3, 4 and 5 mm) in silica gel, decreasing the moisture content to ten percentage points intervals, followed by pre-humidification (100% RH / 24 h) and rehydration. To re-induce DT, germinated seeds were treated for 72 h with PEG (-2.04 MPa) and PEG (-2.04 MPa) + ABA (100 µM) before dehydration. Germinated seeds did not tolerate desiccation to 10% moisture content, irrespectively of the radicle length. However, when incubated in PEG, those with 1 and 2 mm long radicle attained 71% and 29% survival, respectively. The PEG+ABA treatment was efficient to re-establish DT in seeds with 1 mm long radicles (100% survival). The ultrastructural assays of the cells of germinated seeds with 2 and 5 mm length confirmed the obtained physiological results. Germinated seeds of C. fissilis constitute a useful tool for desiccation tolerance investigations. (AU)

FAPESP's process: 05/04139-7 - Carbohydrates of tropical species as modulators of ecophysiological processes and as environmental stress response markers
Grantee:Marcia Regina Braga
Support Opportunities: Research Projects - Thematic Grants