Advanced search
Start date
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photodynamic therapy has antifungal effect and reduces inflammatory signals in Candida albicans-induced murine vaginitis

Full text
Machado-de-Sena, R. M. [1, 2] ; Correa, L. [3] ; Kato, I. T. [1] ; Prates, R. A. [4] ; Senna, A. M. [5, 6] ; Santos, C. C. [1] ; Picanco, D. A. [1] ; Ribeiro, M. S. [1]
Total Authors: 8
[1] IPEN CNEN SP, Ctr Lasers & Applicat, BR-05508000 Sao Paulo - Brazil
[2] IFTO, Fed Inst Educ Sci & Technol, Dept Microbiol & Immunol, BR-77826170 Araguaina, TO - Brazil
[3] Univ Sao Paulo, FOUSP, Sch Dent, Dept Stomatol, BR-05508900 Sao Paulo - Brazil
[4] Nove Julho Univ, UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, BR-01504001 Sao Paulo - Brazil
[5] Palmas Gen Hosp, Dept Oral Surg, BR-77015202 Palmas, TO - Brazil
[6] Tocantinense Inst President Antonio Carlos, Dept Lasers & Photomed, BR-77500000 Porto Nacional, TO - Brazil
Total Affiliations: 6
Document type: Journal article
Source: Photodiagnosis and Photodynamic Therapy; v. 11, n. 3, p. 275-282, SEP 2014.
Web of Science Citations: 15

Background: Vaginal candidiasis (VC) is a disease that affects thousands of women of childbearing age, mainly caused by Candida albicans fungus. Photodynamic therapy (PDT) uses photosensitizing substances that are nontoxic in the dark, but able to produce reactive oxygen species when they are subjected to a light source. In this work our purpose was to investigate PDT effects on fungal burden and inflammatory cells in a murine model of C. albicans-induced vaginal candidiasis. Methods: Female BALB/c mice 6-10 weeks were estrogenized and maintained in this state during all experiment. After 72 h, mices were inoculated intravaginally (IV) with 20 mu L of 2 x 10(5) C. albicans cells suspension. Mice were separated into 5 groups after five days: H (healthy), PBS (control), laser, MB (methylene blue) and PDT. PDT and MB groups received IV 20 mu L solution with 1 mM of MB, others received PBS. PDT and laser groups were irradiated with a red laser (100 mW, 660 nm) in one (36 J, 6 min) or two sessions (18 J, 3 min). After the end of treatment, mice were submitted to microbiological and histomorphometric analysis with ImageJ software. Data were plotted by mean values and standard deviations of CFU/mL and percentage of inflammatory cells area. ANOVA and Bonferroni post-test were used and data were considered significant when p < 0.05. Results: PDT significantly reduced C. albicans after the two tested protocols, however, percentage area of inflammatory cells was significantly reduced just with two sessions of PDT. Conclusions: PDT with MB and red laser is a promising therapy for VC. It is able to reduce fungal infection in biofilm and inflammatory signals associated with VC in a murine model of vaginitis. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 10/13313-9 - Development of methodologies for photodynamic therapy applications on fungal infections
Grantee:Martha Simões Ribeiro
Support type: Regular Research Grants