TGF-beta(1) expression in wound healing is acutely affected by experimental malnutrition and early enteral feeding

Author(s)

Alves, Claudia Cristina ^{[1, 2]} ; Torrinhas, Raquel Susana ^{[1, 2]} ; Giorgi, Ricardo ^[3] ; Brentani, Maria Mitzi ^[4] ; Logullo, Angela Flavia ^[5] ; Waitzberg, Dan Linetzky ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Med Sch FMUSP, Dept Gastroenterol, Lab Nutr & Metab Surg Digest Syst LIM 35, Sao Paulo - Brazil

^[2] Univ Sao Paulo, NAPAN, Food & Nutr Res Ctr, Sao Paulo - Brazil

^[3] FMUSP, Dept Internal Med, Human Nutr & Metab Dis Lab LIM 25, Sao Paulo - Brazil

^[4] FMUSP Sao Paulo, Dept Radiol, Expt Oncol Lab LIM 24, Sao Paulo - Brazil

^[5] Univ Fed Sao Paulo, Paulista Sch Med UNIFESP EPM, Dept Pathol, Sao Paulo - Brazil

• Document type: Journal article
• Source: INTERNATIONAL WOUND JOURNAL; v. 11, n. 5, p. 533-539, OCT 2014.
• Web of Science Citations: 7

Abstract

Malnutrition is associated with the delay or failure of healing. We assessed the effect of experimental malnutrition and early enteral feeding with standard diet or diet supplemented with arginine and antioxidants on the levels of mRNA encoding growth factors in acute, open wound healing. Standardised cutaneous dorsal wounds and gastrostomies for enteral feeding were created in malnourished (M, n = 27) and eutrophic control (E, n = 30) Lewis male adult rats. Both M and E rats received isocaloric and isonitrogenous regimens with oral chow and saline (C), standard (S) or supplemented (A) enteral diets. On post-trauma day 7, mRNA levels of growth factor genes were analysed in wound granulation tissue by reverse transcription polymerase chain reaction (RT-PCR). M(C) rats had significantly lower transforming growth factor (TGF-(1)) mRNA levels than E(C) rats (258 +/- 083 versus 353 +/- 057, P < 001) and in comparison with M(S) and M(A) rats (466 +/- 249 and 461 +/- 211, respectively; P < 005). VEGF and KGF-7 mRNA levels were lower in M(A) rats than in E(A) rats (074 +/- 016 versus 125 +/- 066; and 107 +/- 045 versus 179 +/- 089, respectively; P 004), but did not differ from levels in E(C) and M(C) animals. In experimental open acute wound healing, previous malnutrition decreased local mRNA levels of TGF-(1) genes, which was minimised by early enteral feeding with standard or supplemented diets. (AU)