Evaluation of sequential injection chromatography ... - BV FAPESP
Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of sequential injection chromatography for reversed phase separation of triazine herbicides exploiting monolithic and core-shell columns

Full text
Author(s):
Urio, Ricardo de Pra [1] ; Masini, Jorge C. [1]
Total Authors: 2
Affiliation:
[1] Univ Sao Paulo, Inst Quim, BR-05513970 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Talanta; v. 131, p. 528-534, JAN 2015.
Web of Science Citations: 6
Abstract

This paper describes the development of reversed phase sequential injection chromatography (SIC) methods for separation of simazine (SIM) and atrazine (AT), as well as their metabolites deethylatrazine (DEA), deisopropylatrazine (DIA) and hydroxyatrazine (HAT) exploiting silica based monolithic (50 x 4.6 mm) and core-shell (30 x 4.6 mm, 2.7 mu m particles) columns. The separation was made by stepwise elution with two mobile phases: MP1 composed of 15:85 (v/v) acetonitrile: 2.5 mmol L-1 acetic acid/ammonium acetate buffer (pH 4.2), and MP2, composed of 35:65 (v/v) acetonitrile: 2.5 mmol L-1 acetic acid/ammonium acetate buffer (pH 4.2).The less hydrophobic compounds (DIA, HAT and DEA) eluted with MP1, whereas SIM and AT eluted with MP2. The method using core-shell column exhibited better chromatographic efficiency compared with monolithic column for separation of SIM and AT, but failed to provide base line separation of DIA and HAT. The proposed composition of mobile phases enabled the monolithic column to separate all the studied compounds with resolution > 2.3 at flow rate of 35 mu L s(-1) and sampling throughput of 8 analyses per hour, whereas in the core-shell the maximum flow rate allowed in the SIC system was 8 mu L s(-1) throughput of 3 analyses per hour). The limits of detection were between 24 mu g L-1 (AT) and 40 mu g L-1 (DEA) using the monolithic column, and between 20 mu g L-1 (SIM) and 38 mu g L-1 (DEA) with the core-shell. Ultrasound-assisted extraction (80:20 v/v acetonitrile:water) of a soil sample enriched with the five triazines (250, 500 and 1000 mu g kg(-1)) resulted recoveries between 51% and 121% of the spiked concentrations. (C) 2014 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 11/08309-5 - Development and evaluation of extraction methods and chromatographic separation for determination of triazine, ureas and uracil herbicides in soil and water samples.
Grantee:Ricardo de Prá Urio
Support Opportunities: Scholarships in Brazil - Doctorate