Advanced EPR spectroscopy applied to mechanistic studies on metallodrugs-biomolecu...
| Full text | |
| Author(s): Show less - |
Correia, Isabel
[1]
;
Adao, Pedro
[1]
;
Roy, Somnath
[1]
;
Wahba, Mohamed
[1, 2]
;
Matos, Cristina
[1]
;
Maurya, Mannar R.
[3]
;
Marques, Fernanda
[4]
;
Pavan, Fernando R.
[5]
;
Leite, Clarice Q. F.
[5]
;
Avecilla, Fernando
[6]
;
Pessoa, Joao Costa
[1]
Total Authors: 11
|
| Affiliation: | [1] Univ Lisbon, Inst Super Tecn, Ctr Quim Estrutural, P-1049001 Lisbon - Portugal
[2] Natl Res Ctr, Inorgan Chem Dep, Cairo - Egypt
[3] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh - India
[4] Univ Lisbon, Inst Super Tecn, Ctr Ciencias & Tecnol Nucl, P-2695066 Bobadela Lrs - Portugal
[5] UNESP, Fac Ciencias Farmaceut, BR-14801902 Araraquara, SP - Brazil
[6] Univ A Coruna, Dept Quim Fundamental, La Coruna 15071 - Spain
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | Journal of Inorganic Biochemistry; v. 141, p. 83-93, DEC 2014. |
| Web of Science Citations: | 48 |
| Abstract | |
Several mixed ligand vanadium and copper complexes were synthesized containing 8-hydroxyquinoline (8HQ) and a ligand such as picolinato (pic(-)), dipicolinato (dipic(2-)) or a Schiff base. The complexes were characterized by spectroscopic techniques and by single-crystal X-ray diffraction in the case of {[}(VO)-O-V(L-pheolnaph-im)(5-Cl-8HQ)] and {[}(VO)-O-V(OMe)(8HQ)(2)], which evidenced the distorted octahedral geometry of the complexes. The electronic absorption data showed the presence of strong ligand to metal charge transfer bands, significant solvent effects, and methoxido species in methanol, which was further confirmed by V-51- NMR spectroscopy. The structures of {[}Cu-II(dipic)(8HQ)]Na and {[}(VO)-O-IV(pic)(8HQ)] were confirmed by EPR spectroscopy, showing only one species in solution. The biological activity of the compounds was assessed through the minimal inhibitory concentration (MIC) of the compounds against Mycobacterium tuberculosis (Mtb) and the cytotoxic activity against the cisplatin sensitive/resistant ovarian cells A2780/A2780cisR and the non-tumorigenic HEK cells (IC50 values). Almost all tested vanadium complexes were very active against Mtb and the MICs were comparable to, or better than, the MICs of drugs, such as streptomycin. The activity of the complexes against the A2780 cell line was dependent on incubation time presenting IC50 values in the 3-14 mu M (at 48 h) range. In these conditions, the complexes were significantly ({*}P < 0.05-{*}{*}P < 0.001) more active than cisplatin (22 mu M), in the A2780 cells and even surpassing its activity in the cisplatin-resistant cells A2780cisR (2.4-8 mu M vs. 75.4; {*}{*}P < 0.001). In the non-tumorigenic HEK cells poor selectivity toward cancer cells for most of the complexes was observed, as well as for cisplatin. (C) 2014 Elsevier Inc. All rights reserved. (AU) | |
| FAPESP's process: | 13/14957-5 - Research potential against tuberculosis of a new class of furoxan compounds and nanostructured compounds of the ruthenium(II) and copper (II) |
| Grantee: | Fernando Rogério Pavan |
| Support type: | Research Grants - Young Investigators Grants |