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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Photobiostimulation reduces edema formation induced in mice by Lys-49 phospholipases A(2) isolated from Bothrops moojeni venom

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Author(s):
Nadur-Andrade, Nikele [1] ; Dale, Camila Squarzone [2] ; dos Santos, Adriano Silvio [1] ; Soares, Andreimar M. [3] ; de Lima, Carlos J. [4] ; Zamuner, Stella Regina [1]
Total Authors: 6
Affiliation:
[1] Univ Nove Julho, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Anat, Sao Paulo - Brazil
[3] Fed Univ Rondonia, Oswaldo Cruz Fdn, BR-76812245 Porto Velho, RO - Brazil
[4] Univ Camilo Castelo Branco, Lab Biomed Instrumentat, Sao Jose Dos Campos - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES; v. 13, n. 11, p. 1561-1567, 2014.
Web of Science Citations: 8
Abstract

The prominent local myotoxic effects induced by Bothrops snake venom are due, in part, to myotoxins. This effect is not neutralized by antivenom, which is the main therapy for victims of snakebite. Two basic myotoxins named MjTX-I and MjTX-II were isolated from Bothrops moojeni venom. Both myotoxins have a Lys-49 phospholipase A(2) structure devoid of enzymatic activity, but are highly myonecrotic and edema-inducing. In this study, we analyzed the effect of a low-level laser (LLL) at 685 nm, an energy density of 2.2 J cm(-2), and the irradiation time of 15 s, and a light emitting diode (LED) at 635 or 945 nm at energy densities of 4 and 3.8 J cm(-2), and irradiation times of 41 and 38 s, respectively, applied 30 min and 3 h after edema formation in mice caused by MjTX-I or MjTX-II. MjTX-I or MjTX-II caused a significant edema formation in envenomed paws. LLL and LED irradiation significantly reduced the edema formation by both myotoxins from 1 up to 6 hours after the injection. Both LLL and LEDs were similar in reducing the edema formation induced by myotoxins. The combined photobiostimulation with antivenom had the same effect in reducing edema as treatment with the LLL or LEDs alone. In conclusion, the results of this study indicate that photobiostimulation could be used in association with antivenom therapy for treatment of local effects of Bothrops species venom. (AU)

FAPESP's process: 12/09710-8 - Effect of photobiostimulation on the local effect induced by Bothrops moojeni and myotoxins isolated.
Grantee:Nikele Nadur Andrade
Support Opportunities: Scholarships in Brazil - Doctorate