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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Species-specific PCR for the identification of Cooperia curticei (Nematoda: Trichostrongylidae) in sheep

Full text
Author(s):
Amarante, M. R. V. [1] ; Bassetto, C. C. [1] ; Neves, J. H. [1] ; Amarante, A. F. T. [1]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Parasitol, BR-18618970 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: JOURNAL OF HELMINTHOLOGY; v. 88, n. 4, p. 447-452, DEC 2014.
Web of Science Citations: 2
Abstract

Agricultural ruminants usually harbour mixed infections of gastrointestinal nematodes. A specific diagnosis is important because distinct species can differ significantly in their fecundity and pathogenicity. Haemonchus spp. and Cooperia spp. are the most important gastrointestinal nematodes infecting ruminants in subtropical/tropical environments. In Brazil, C. punctata is more adapted to cattle than sheep. Additionally, C. spatulata appears to be more adapted to cattle, whereas C. curticei is more adapted to sheep. However, infection of sheep with C. punctata is common when cattle and sheep share the same pasture. Although morphological analyses have been widely used to identify nematodes, molecular methods can overcome technical limitations and help improve species-specific diagnoses. Genetic markers in the first and second internal transcribed spacers (ITS-1 and ITS-2, respectively) of nuclear ribosomal DNA (rDNA) have been used successfully to detect helminths. In the present study, the ITS-1 region was analysed and used to design a species-specific oligonucleotide primer pair to identify C. curticei. The polymerase chain reaction (PCR) product was sequenced and showed 97% similarity to C. oncophora partial ITS-1 clones and 99% similarity to the C. curticei sequence JF680982. The specificity of this primer pair was corroborated by the analysis of 17 species of helminths, including C. curticei, C. punctata and C. spatulata. Species-specific diagnosis, which has implications for rapid and reliable identification, can support studies on the biology, ecology and epidemiology of trichostrongylid nematodes in a particular geographical location. (AU)

FAPESP's process: 10/18678-5 - Protection of sheep and cattle against haemoncose after immunization with antigens from the intestinal membrane of Haemonchus contortus.
Grantee:César Cristiano Bassetto
Support Opportunities: Scholarships in Brazil - Doctorate