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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Streptomyces araujoniae Produces a Multiantibiotic Complex with Ionophoric Properties to Control Botrytis cinerea

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Author(s):
Silva, Leonardo Jose [1] ; Crevelin, Eduardo Jose [2] ; Souza, Wallace Rafael [1] ; Beraldo Moraes, Luiz Alberto [2] ; Melo, Itamar Soares [1] ; Zucchi, Tiago Domingues [1]
Total Authors: 6
Affiliation:
[1] EMBRAPA, Lab Microbiol Ambiental, Jaguariuna - Brazil
[2] Univ Sao Paulo, Lab Espectrometria Massa Aplicado Quim Prod Nat, FFCLRP, Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: PHYTOPATHOLOGY; v. 104, n. 12, p. 1298-1305, DEC 2014.
Web of Science Citations: 7
Abstract

A recently described actinomycete species (Streptomyces araujoniae ASBV-1T) is effective against many phytopathogenic fungi. In this study, we evaluated the capacity of this species to inhibit Botrytis cinerea development in strawberry pseudofruit, and we identified the chemical structures of its bioactive compounds. An ethyl acetate crude extract (0.1 mg ml(-1)) of ASBV-1T fermentation broth completely inhibited fungus growth in strawberry pseudofruit under storage conditions. The crude extract was fractionated by preparative high-performance liquid chromatography; the active fraction was further evaluated by tandem mass spectrometry. ASBV-1T produced a multiantibiotic complex with ionophoric properties. This complex contained members of the macrotetralides class (including monactin, dinactin, trinactin, and tetranactin) and the cyclodepsipeptide valinomycin, all of which were active against B. cinerea. Furthermore, the addition of 2 mM MgSO4 and 1 mM ZnSO4 enhanced macrotetralide and valinomycin production, respectively, in the culture broth. These compounds are considered to be the main active molecules that S. araujoniae produces to control B. cinerea. Their low to moderate toxicity to humans and the environment justifies the application of ASBV-1T in biological control programs that aim to mitigate the damage caused by this phytopathogen. (AU)

FAPESP's process: 11/14333-6 - Diversity and polyphasic taxonomy of actinobacteria isolated from reforestation areas
Grantee:Tiago Domingues Zucchi
Support Opportunities: Scholarships in Brazil - BIOTA - Young Researchers