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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Toll-Like Receptor 2 and 4 RNA Expression and the Cytokine Profile in Postmenopausal Women with Metabolic Syndrome

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Orsatti, Claudio Lera [1] ; Petri Nahas, Eliana Aguiar [1] ; Nahas-Neto, Jorge [1] ; Orsatti, Fabio Lera [2] ; Giorgi, Vanessa Innocenti [3] ; Witkin, Steven S. [4]
Total Authors: 6
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP - Brazil
[2] Inst Hlth Sci UFTM, Dept Sports Sci, Uberaba, MG - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[4] Weill Cornell Med Coll, Div Immunol & Infect Dis, Dept Obstet & Gynecol, New York, NY - USA
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 9, n. 10 OCT 17 2014.
Web of Science Citations: 4

Objective: To evaluate the gene expression of Toll-Like (TLR-2 and TLR-4) receptors and cytokine profile in postmenopausal women with or without metabolic syndrome (MetS). Methods: In this cross-sectional study, 311 Brazilian women (age >= 45 years and amenorrhea >= 12 months) were included. Women showing three or more of the following diagnostic criteria were diagnosed as positive for MetS: waist circumference>88 cm, triglycerides >= 150 mg/dL, HDL cholesterol<50 mg/dL, blood pressure >= 130/85 mmHg, and fasting glucose >= 100 mg/dL. The expression of TLR-2 and TLR-4 in peripheral blood was evaluated by RNA extraction and subsequent real time PCR analysis. The cytokine profile, tumor necrosis factor alpha (TNF-alpha) and interleukins 1 beta, 6, and 10, were measured by ELISA. Results: The expression of TLR-2 RNA was demonstrated in 32.5% and TLR-4 in 20.6% of the subjects. There was no association between the expression of TLR-2 and TLR-4 and the presence or absence of MetS (P>0.05). A greater production of IL-6 was associated with TLR-2 and TLR-4 expressions and greater production of TNF-alpha was associated only with TLR-2 expression (P>0.05). Only the lower quartile of IL-10 was associated with the presence of the MetS (P>0.05). Conclusions: TLR-2 and TLR-4 expressions were associated with increased pro-inflammatory cytokines, IL-6 and TNF-alpha, with no association with biomarkers of MetS. The low concentrations of IL-10 may suggest an anti-inflammatory modulation in postmenopausal women with MetS. (AU)

FAPESP's process: 09/14884-2 - Evaluation of early biomarkers of cardiovascular disease - clinic, inflammatory and ultrasonographic - in postmenopausal women
Grantee:Eliana Aguiar Petri Nahás
Support type: Regular Research Grants
FAPESP's process: 09/18256-6 - Evaluation of genetic polymorphism of mannose-binding lectin, the gene expression of Toll-like receptors (TLR)and cytokine profile as bio-markers of cardiovascular risk in postmenopausal women
Grantee:Cláudio Lera Orsatti
Support type: Scholarships in Brazil - Doctorate