Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of Toll-Like Receptor 2 and 4 RNA Expression and the Cytokine Profile in Postmenopausal Women with Metabolic Syndrome

Full text
Author(s):
Orsatti, Claudio Lera [1] ; Petri Nahas, Eliana Aguiar [1] ; Nahas-Neto, Jorge [1] ; Orsatti, Fabio Lera [2] ; Giorgi, Vanessa Innocenti [3] ; Witkin, Steven S. [4]
Total Authors: 6
Affiliation:
[1] Sao Paulo State Univ UNESP, Botucatu Med Sch, Dept Gynecol & Obstet, Botucatu, SP - Brazil
[2] Inst Hlth Sci UFTM, Dept Sports Sci, Uberaba, MG - Brazil
[3] Sao Paulo State Univ UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[4] Weill Cornell Med Coll, Div Immunol & Infect Dis, Dept Obstet & Gynecol, New York, NY - USA
Total Affiliations: 4
Document type: Journal article
Source: PLoS One; v. 9, n. 10 OCT 17 2014.
Web of Science Citations: 4
Abstract

Objective: To evaluate the gene expression of Toll-Like (TLR-2 and TLR-4) receptors and cytokine profile in postmenopausal women with or without metabolic syndrome (MetS). Methods: In this cross-sectional study, 311 Brazilian women (age >= 45 years and amenorrhea >= 12 months) were included. Women showing three or more of the following diagnostic criteria were diagnosed as positive for MetS: waist circumference>88 cm, triglycerides >= 150 mg/dL, HDL cholesterol<50 mg/dL, blood pressure >= 130/85 mmHg, and fasting glucose >= 100 mg/dL. The expression of TLR-2 and TLR-4 in peripheral blood was evaluated by RNA extraction and subsequent real time PCR analysis. The cytokine profile, tumor necrosis factor alpha (TNF-alpha) and interleukins 1 beta, 6, and 10, were measured by ELISA. Results: The expression of TLR-2 RNA was demonstrated in 32.5% and TLR-4 in 20.6% of the subjects. There was no association between the expression of TLR-2 and TLR-4 and the presence or absence of MetS (P>0.05). A greater production of IL-6 was associated with TLR-2 and TLR-4 expressions and greater production of TNF-alpha was associated only with TLR-2 expression (P>0.05). Only the lower quartile of IL-10 was associated with the presence of the MetS (P>0.05). Conclusions: TLR-2 and TLR-4 expressions were associated with increased pro-inflammatory cytokines, IL-6 and TNF-alpha, with no association with biomarkers of MetS. The low concentrations of IL-10 may suggest an anti-inflammatory modulation in postmenopausal women with MetS. (AU)

FAPESP's process: 09/14884-2 - Evaluation of early biomarkers of cardiovascular disease - clinic, inflammatory and ultrasonographic - in postmenopausal women
Grantee:Eliana Aguiar Petri Nahás
Support type: Regular Research Grants
FAPESP's process: 09/18256-6 - Evaluation of genetic polymorphism of mannose-binding lectin, the gene expression of Toll-like receptors (TLR)and cytokine profile as bio-markers of cardiovascular risk in postmenopausal women
Grantee:Cláudio Lera Orsatti
Support type: Scholarships in Brazil - Doctorate