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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Mechanical properties of acellular mouse lungs after sterilization by gamma irradiation

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Author(s):
Uriarte, Juan J. [1, 2, 3] ; Nonaka, Paula N. [1] ; Campillo, Noelia [1, 2, 4] ; Palma, Renata K. [1] ; Melo, Esther [1, 2, 3] ; de Oliveira, Luis V. F. [5] ; Navajas, Daniel [1, 2, 4] ; Farre, Ramon [1, 2, 3]
Total Authors: 8
Affiliation:
[1] Univ Barcelona, Fac Med, Unitat Biofis & Bioengn, Barcelona 7 - Spain
[2] CIBER Enfermedades Resp, Bunyola - Spain
[3] Inst Invest Biomed August Pi Sunyer, Barcelona - Spain
[4] Inst Bioengn Catalunya, Barcelona - Spain
[5] Nove de Julho Univ, Masters & Doctoral Degree Programs Rehabil Sci, Sao Paulo - Brazil
Total Affiliations: 5
Document type: Journal article
Source: JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS; v. 40, p. 168-177, DEC 2014.
Web of Science Citations: 18
Abstract

Lung bioengineering using decellularized organ scaffolds is a potential alternative for lung transplantation. Clinical application will require donor scaffold sterilization. As gamma-irradiation is a conventional method for sterilizing tissue preparations for clinical application, the aim of this study was to evaluate the effects of lung scaffold sterilization by gamma irradiation on the mechanical properties of the acellular lung when subjected to the artificial ventilation maneuvers typical within bioreactors. Twenty-six mouse lungs were decellularized by a sodium dodecyl sulfate detergent protocol. Eight lungs were used as controls and 18 of them were submitted to a 31 kGy gamma irradiation sterilization process (9 kept frozen in thy ice and 9 at room temperature). Mechanical properties of acellular lungs were measured before and after irradiation. Lung resistance (R-L) and elastance (E-L) were computed by linear regression fitting of recorded signals during mechanical ventilation (tracheal pressure, flow and volume). Static (E-st) and dynamic (E-dyn) elastances were obtained by the end-inspiratory occlusion method. After irradiation lungs presented higher values of resistance and elastance than before irradiation: R-L increased by 41.1% (room temperature irradiation) and 32.8% (frozen irradiation) and E-L increased by 41.8% (room temperature irradiation) and 31.8% (frozen irradiation). Similar increases were induced by irradiation in E-st and E-dyn. Scanning electron microscopy showed slight structural changes after irradiation, particularly those kept frozen. Sterilization by gamma irradiation at a conventional dose to ensure sterilization modifies acellular lung mechanics, with potential implications for lung bioengineering. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 11/21709-2 - Resistance and compliance of decellularized lung scaffolds along recellularization with stem cells.
Grantee:Paula Naomi Nonaka
Support Opportunities: Scholarships in Brazil - Doctorate