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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Reduced susceptibility to vancomycin and biofilm formation in methicillin-resistant Staphylococcus epidermidis isolated from blood cultures

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Author(s):
Pinheiro, Luiza [1] ; Brito, Carla Ivo [1] ; Pereira, Valeria Cataneli [1] ; de Oliveira, Adilson [1] ; Camargo, Carlos Henrique [1] ; Ribeiro de Souza da Cunha, Maria de Lourdes [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Microbiol Lab, Dept Microbiol & Imunol, Inst Biociencias Botucatu, Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: Memórias do Instituto Oswaldo Cruz; v. 109, n. 7, p. 871-878, NOV 2014.
Web of Science Citations: 13
Abstract

This study aimed to correlate the presence of ica genes, biofilm formation and antimicrobial resistance in 107 strains of Staphylococcus epidermidis isolated from blood cultures. The isolates were analysed to determine their methicillin resistance, staphylococcal cassette chromosome mec (SCCmec) type, ica genes and biofilm formation and the vancomycin minimum inhibitory concentration (MIC) was measured for isolates and subpopulations growing on vancomycin screen agar. The mecA gene was detected in 81.3% of the S. epidermidis isolated and 48.2% carried SCCmec type III. The complete icaADBC operon was observed in 38.3% of the isolates; of these, 58.5% produced a biofilm. Furthermore, 47.7% of the isolates grew on vancomycin screen agar, with an increase in the MIC in 75.9% of the isolates. Determination of the MIC of subpopulations revealed that 64.7% had an MIC ≥ 4 μg mL-1, including 15.7% with an MIC of 8 μg mL-1 and 2% with an MIC of 16 μg mL-1. The presence of the icaADBC operon, biofilm production and reduced susceptibility to vancomycin were associated with methicillin resistance. This study reveals a high level of methicillin resistance, biofilm formation and reduced susceptibility to vancomycin in subpopulations of S. epidermidis. These findings may explain the selection of multidrug-resistant isolates in hospital settings and the consequent failure of antimicrobial treatment. (AU)

FAPESP's process: 10/18720-1 - Staphylococcus epidermidis: virulence and antimicrobial resistance in isolates from blood cultures
Grantee:Luiza Hubinger
Support Opportunities: Scholarships in Brazil - Scientific Initiation