Effect of Avocado/Soybean Unsaponifiables on Osseointegration: A Proof-of-Principle Preclinical In Vivo Study

Author(s)

Pimentel Lopes de Oliveira, Guilherme Jose ^[1] ; Freitas de Paula, Luiz Guilherme ^[1] ; Spin-Neto, Rubens ^[2] ; Stavropoulos, Andreas ^[3] ; Spolidorio, Luis Carlos ^[1] ; Marcantonio, Jr., Elcio ^[1] ; Chierici Marcantonio, Rosemary Adriana ^[1]

Affiliation

^[1] Univ Estadual Paulista, Araraquara Dent Sch, Fac Dent, Sao Paulo - Brazil

^[2] Aarhus Univ, Dept Dent, Sect Oral Radiol, Aarhus - Denmark

^[3] Malmo Univ, Fac Odontol, Dept Periodontol, Malmo - Sweden

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS; v. 29, n. 4, p. 949-957, JUL-AUG 2014.
• Web of Science Citations: 4

Abstract

Purpose: To evaluate the influence of administration of avocado/soybean unsaponifiables (ASU) on implant osseointegration in rat tibiae. Materials and Methods: Thirty rats were randomly assigned into one of three equal-sized groups: (1) ASU1: administration of ASU starting 7 days prior to implant placement; (2) ASU2: administration of ASU starting on the day of implant placement, and (3) CTL: administration of saline solution. In all animals, one titanium implant was placed in each tibia. All animals received ASU or saline solution by gavage daily until sacrifice 60 days postoperatively. Implant osseointegration and bone maturation were assessed by biomechanical and radiographic bone density analysis; descriptive histology; immunohistochemistry for bone morphogenetic protein 2 (BMP-2), transforming growth factor beta 1 (TGF-beta 1), and osteocalcin; and histomorphometric evaluation of bone-to-implant contact (BIC) and mineralized bone area fraction within the threads of the implant. Results: ASU1 and ASU2 showed three times higher expression of BMP-2 and nine times higher expression of TGF-beta compared with CTL (P < .05). Histomorphometric analysis, however, showed that both ASU1 and ASU2 groups presented significantly higher BIC values only in the cortical bone compartment when compared to CTL (P < .05). Conclusion: ASU consumption seems to exert only a subtle effect on implant osseointegration. (AU)

• FAPESP's process: 10/13029-9 - Effect of avocado and soybean unsaponifiables on induced periodontal disease and osseointegration. Histological, radiological and immunohistochemistry in rats.
• Grantee: Rosemary Adriana Chierici Marcantonio
• Support Opportunities: Regular Research Grants
• FAPESP's process: 09/17395-2 - Influence of avocado and soybean unsaponifiables in the induced periodontal disease and bone around integrated dental implants. Histological, radiographic and immuno-inflammatory analysis in rats.
• Grantee: Guilherme José Pimentel Lopes de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate