| Full text | |
| Author(s): |
Zsoegoen, Agustin
[1]
;
Alves Negrini, Ana Clarissa
[2, 3]
;
Pereira Peres, Lazaro Eustaquio
[1]
;
Nguyen, Hoa Thi
[4]
;
Ball, Marilyn C.
[4]
Total Authors: 5
|
| Affiliation: | [1] Univ Sao Paulo, Dept Ciencias Biol, Escola Super Agr Luiz de Queiroz, BR-13418900 Piracicaba, SP - Brazil
[2] Embrapa Hortalicas, BR-70359970 Brasilia, DF - Brazil
[3] Australian Natl Univ, Res Sch Biol, Plant Sci Div, Canberra, ACT 0200 - Australia
[4] Hoa Thi Nguyen, Australian Natl Univ, Res Sch Biol, Plant Sci Div, Canberra, ACT 0200 - Australia
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | NEW PHYTOLOGIST; v. 205, n. 2, p. 618-626, JAN 2015. |
| Web of Science Citations: | 17 |
| Abstract | |
Bundle sheath extensions (BSEs) are key features of leaf structure whose distribution differs among species and ecosystems. The genetic control of BSE development is unknown, so BSE physiological function has not yet been studied through mutant analysis. We screened a population of ethyl methanesulfonate (EMS)-induced mutants in the genetic background of the tomato (Solanum lycopersicum) model Micro-Tom and found a mutant lacking BSEs. The leaf phenotype of the mutant strongly resembled the tomato mutant obscuravenosa (obv). We confirmed that obv lacks BSEs and that it is not allelic to our induced mutant, which we named obv-2. Leaves lacking BSEs had lower leaf hydraulic conductance and operated with lower stomatal conductance and correspondingly lower assimilation rates than wild-type leaves. This lower level of function occurred despite similarities in vein density, midvein vessel diameter and number, stomatal density, and leaf area between wild-type and mutant leaves, the implication being that the lack of BSEs hindered water dispersal within mutant leaves. Our results comparing near-isogenic lines within a single species confirm the hypothesised role of BSEs in leaf hydraulic function. They further pave the way for a genetic model-based analysis of a common leaf structure with deep ecological consequences. (AU) | |
| FAPESP's process: | 13/11541-2 - Interaction between auxin and the florigen gene family in the control of architecture and yield in tomato (Solanum lycopersicum L.) |
| Grantee: | Agustin Zsögön |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |