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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Hatching, survival and deformities of piracanjuba (Brycon orbignyanus) embryos subjected to different cooling protocols

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Author(s):
Faria Paes, Maria do Carmo [1] ; da Silva, Regiane Cristina [1] ; do Nascimento, Nivaldo Ferreira [1] ; Valentin, Fernanda Nogueira [1] ; Senhorini, Jose Augusto [2] ; Okada Nakaghi, Laura Satiko [1]
Total Authors: 6
Affiliation:
[1] Univ Estadual Paulista, Aquaculture Ctr, BR-14884900 Sao Paulo - Brazil
[2] CEPTA ICMBio, Natl Ctr Res & Continental Fish Conservat, BR-13630970 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: CRYOBIOLOGY; v. 69, n. 3, p. 451-456, DEC 2014.
Web of Science Citations: 4
Abstract

Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0 +/- 2.0 degrees C and 8.0 +/- 2.0 degrees C) and refrigeration times (6, 10, 24, 72 and 168 h), with the exception of the positive control (incubation without previous cooling). At the end of each refrigeration time, the embryos were acclimatized, rehydrated and incubated to determine hatching, survival and deformity rates. Morphological analysis of embryos was also carried out. The results showed that temperature and refrigeration time are critical factors for embryo survival. No embryos survived after 24, 72 and 168 h of refrigeration. Furthermore, when the refrigeration time increased from 6 to 10 h and the temperature decreased from 8.0 +/- 2.0 degrees C to 0.0 +/- 2.0 degrees C, mortality rates increased significantly. It was also found that in all protocols dead eggs and/or larvae with some degree of deformity were present. The main larval deformities observed were the malformation of the head, tail, yolk sac, vertebral column and eyes. (C) 2014 Elsevier Inc. All rights reserved. (AU)

FAPESP's process: 09/12340-5 - Microscopy and viability of embryos of piracanjuba (Brycon orbignyanus) submitted to different cryoprotectants, temperature and time of storage
Grantee:Maria do Carmo Faria Paes
Support Opportunities: Scholarships in Brazil - Doctorate