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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antitumoural activity of Brazilian red propolis fraction enriched with xanthochymol and formononetin: An in vitro and in vivo study

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Author(s):
Novak, Estela Maria [1, 2, 3] ; Costa Silva, Martha Silveira e [1] ; Marcucci, Maria Cristina [4] ; Helena Frankland Sawaya, Alexandra Christine [5] ; Lopez, Begona Gimenez-Cassina [5] ; Henriques Zanella Fortes, Maria Angela [6] ; Giorgi, Ricardo Rodrigues [6] ; Marumo, Kamila Tamie [7] ; Rodrigues, Rosangela Felipe [8] ; Maria, Durvanei Augusto [8]
Total Authors: 10
Affiliation:
[1] Fundacao Prosangue Hemoctr Sao Paulo, BR-05403000 Sao Paulo - Brazil
[2] Lab Invest Med LIM 36 ICR HCFMUSP, Sao Paulo - Brazil
[3] Inst Tratamento Canc ITACI ICR HCFMUSP, Sao Paulo - Brazil
[4] Univ Bandeirante Anhanguera Sao Paulo, Grad Program Biotechnol & Pharm, Sao Paulo, SP - Brazil
[5] State Univ Campinas UNICAMP, Biosci & Technol Bioact Prod Postgrad Program, Dept Plant Biol, Inst Biol, Campinas, SP - Brazil
[6] Univ Sao Paulo, Fac Med, LIM 25, Cellular & Mol Endocrinol Lab, Sao Paulo, SP - Brazil
[7] Butantan Inst, Biochem & Biophys Lab, Sao Paulo - Brazil
[8] Univ Sao Paulo, Fac Vet Med & Anim Sci, Dept Surg, Sao Paulo, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: Journal of Functional Foods; v. 11, p. 91-102, NOV 2014.
Web of Science Citations: 15
Abstract

In this study, ultra-high performance liquid chromatography-mass spectrometry and tandem mass spectrometry of selected ions were used to characterise the ethanolic extract of a sample of Brazilian red propolis (EEBRP) and an active fraction (BRP-IV) containing xanthochymol and formononetin. The antiproliferative effect of BRP-IV was assayed using melanoma tumour xenografts in mice and HL-60, K562, RPMI8226, B16F10 cell lines. This fraction inhibited growth of tumour cell lines with IC50 values of 20.5 +/- 2.4 to 32.6 +/- 2.6 mu g/mL while EEBRP induced cytotoxic effect with IC50 values of 29.7 +/- 1.5 to 42.1 +/- 8.7 mu g/mL. BRP-IV also inhibited the proliferation of B16F10 cells by blocking cell cycle progression in the G2/M phase and inducing apoptosis. Administration of 10 mg/kg/day BRP-IV suppressed the growth of B16F0 tumour xenografts in C57BL/6 mice with less general toxicity than control groups. Taken together, these results indicate that BRP-IV can be considered a promising anticancer drug for the treatment of human cancers. (C) 2014 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/03091-4 - Analysis of the composition of samples of red Brazilian propolis by mass spectrometry with electrospray ionization and ultra high efficiency liquid chromatography (UPLC-ESI-MS) and evaluation of the antioxidant and antimicrobial activity.
Grantee:Begona Gimenez-Cassina Lopez
Support Opportunities: Scholarships in Brazil - Master