| Full text | |
| Author(s): |
Goncalves, Viviane Martinelli
[1]
;
D'Almeida, Vania
[2]
;
Mueller, Karen Barbosa
[3]
;
Real, Fernando
[1]
;
Mortara, Renato Arruda
[1]
Total Authors: 5
|
| Affiliation: | [1] Univ Fed Sao Paulo, Escola Paulista Med, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo, Escola Paulista Med, Dept Psychobiol, BR-04023062 Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Escola Paulista Med, Dept Pediat, BR-04023062 Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Microbes and Infection; v. 16, n. 3, p. 253-260, MAR 2014. |
| Web of Science Citations: | 2 |
| Abstract | |
Lysosomal integral membrane protein 2 (LDM1P-2, SCARB2) is directly linked to beta-glucocerebrosidase enzyme (beta GC) and mediates the transport of this enzyme from the Golgi complex to lysosomes. Active beta GC cleaves the beta-glycosidic linkages of glucosylceramide, an intermediate in the metabolism of sphingoglycolipids, generating ceramide. In this study we used mouse embryonic fibroblasts (MEFs) deficient for LIMP-2 and observed that these cells were more susceptible to infection by extracellular amastigotes of the protozoan parasite Trypanosoma cruzi when compared to wild-type (WT) fibroblasts. The absence of LIMP-2 decreases the activity of beta GC measured in fibroblast extracts. Replacement of beta GC enzyme in LIMP-2 deficient fibroblasts restores the infectivity indices to those of WT cells in T cruzti invasion assays. Considering the participation of beta GC in the production of host cell ceramide, we propose that T cruzi extracellular amastigotes are more invasive to cells deficient in this membrane component. These results contribute to our understanding of the role of host cell lysosomal components in T cruzi invasion. (C) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved. (AU) | |
| FAPESP's process: | 10/19335-4 - Experimental study on the mechanisms of fusion between heterotypic Leishmania spp. parasitophorous vacuoles |
| Grantee: | Fernando Roberto Oliveira Real |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |